SSH1 CRISPR/Cas9 KO Plasmid (m): sc-433087
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSH1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SSH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSH1 CRISPR/Cas9 KO Plasmid (m) | sc-433087 | 20 µg | $397.00 |
Overview
Slingshot protein phosphatase 1 (SSH1) is a cofilin phosphatase that regulates actin filament turnover by dephosphorylating and activating ADF/cofilin, thereby promoting F-actin severing and cytoskeletal remodeling. In mouse cells, SSH1 integrates signals from Rho-family GTPases and kinases such as LIMK to control lamellipodia dynamics, cell polarity, adhesion, and directional migration. This actin-regulatory axis influences endocytosis, neurite outgrowth, and mechanotransduction, linking SSH1 activity to processes relevant to development, immune cell trafficking, and tissue remodeling. Dysregulation of cofilin–LIMK–SSH signaling has been associated with altered invasive behavior and stress responses in multiple disease-relevant contexts, making Ssh1 a useful target for pathway interrogation.
SSH1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ssh1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ssh1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ssh1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SSH1 protein expression.
This CRISPR knockout system enables efficient generation of Ssh1-deficient cell models for investigation of SSH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ssh1 exon(s) critical for SSH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ssh1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SSH1 CRISPR/Cas9 KO Plasmid (m) and SSH1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ssh1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SSH1 HDR Plasmid (m) and SSH1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ssh1 homology arms to support homology-directed repair at defined Ssh1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.