SSEA-3 Antibody (MC-631): sc-73593

SSEA-3 Antibody (MC-631) is a rat monoclonal IgM antibody that detects SSEA-3 in mouse, rat, and human samples through immunofluorescence (IF) and flow cytometry (FCM) applications. SSEA-3, or Stage Specific Embryonic Antigen-3, plays a crucial role in identifying and characterizing undifferentiated embryonic stem cells and embryonal carcinoma cells, serving as a marker for pluripotency. This glycosylated protein is primarily located on the cell surface, where SSEA-3 is involved in cell-cell interactions and signaling pathways that maintain the undifferentiated state of stem cells. SSEA-3 is essential for the self-renewal and pluripotency of these cells, making SSEA-3 a vital component in developmental biology and regenerative medicine research. Additionally, SSEA-3 expression is upregulated in response to certain stimuli, such as interferon, which can enhance the potential for stem cell differentiation without compromising cell viability. Anti-SSEA-3 antibody (MC-631) enables researchers to effectively study the dynamics of stem cell behavior and the mechanisms underlying cell differentiation, providing insights that are critical for advancements in therapeutic applications.

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SSEA-3 Antibody (MC-631) References:

1. The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency.  |  Brimble, SN., et al. 2007. Stem Cells. 25: 54-62. PMID: 17008424
2. Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo.  |  Williams, SA. and Stanley, P. 2009. Glycoconj J. 26: 335-47. PMID: 18773292
3. SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers.  |  Suzuki, Y., et al. 2013. Int J Oncol. 42: 161-7. PMID: 23175153
4. Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.  |  Kinoshita, K., et al. 2015. Stem Cells Transl Med. 4: 146-55. PMID: 25561682
5. Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells.  |  Cheung, SK., et al. 2016. Proc Natl Acad Sci U S A. 113: 960-5. PMID: 26677875
6. Possibility of inhibiting arthritis and joint destruction by SSEA-3 positive cells derived from synovial tissue in rheumatoid arthritis.  |  Kurose, R., et al. 2017. Regen Ther. 7: 82-88. PMID: 30271856
7. Development of Fluorescently Labeled SSEA-3, SSEA-4, and Globo-H Glycosphingolipids for Elucidating Molecular Interactions in the Cell Membrane.  |  Asano, S., et al. 2019. Int J Mol Sci. 20: PMID: 31817926
8. A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood.  |  Sato, T., et al. 2020. Cell Transplant. 29: 963689720923574. PMID: 32525407
9. SSEA-3 and 4 are not essential for the induction or properties of mouse iPS cells.  |  Hamamura, K., et al. 2020. J Oral Sci. 62: 393-396. PMID: 32684574
10. Role of glycosphingolipid SSEA-3 and FGF2 in the stemness and lineage commitment of multilineage differentiating stress enduring (MUSE) cells.  |  Aprile, D., et al. 2023. Cell Prolif. 56: e13345. PMID: 36225120

The preferred SSEA-3 antibody is SSEA-3 Antibody (631): sc-21703.