SSEA-3 Antibody (631): sc-21703

SSEA-3 Antibody (631) is a rat monoclonal IgM antibody that detects SSEA-3 in mouse, rat, and human samples by immunofluorescence (IF) and flow cytometry (FCM). Anti-SSEA-3 antibody (631) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SSEA-3 plays a crucial role in identifying and characterizing pluripotent stem cells, serving as a key marker for undifferentiated embryonic stem cells and embryonal carcinoma cells. SSEA-3 is primarily located on the cell surface, where SSEA-3 contributes to maintaining the undifferentiated state of stem cells, allowing proliferation while retaining differentiation potential into various cell types. SSEA-3 expression is essential for isolating and studying stem cells by distinguishing undifferentiated from differentiated cells, supporting research in developmental biology and regenerative medicine. SSEA-3 also participates in cell-cell interactions and signaling pathways vital for stem cell maintenance and differentiation, making SSEA-3 an important target for understanding stem cell biology and potential therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SSEA-3 Antibody (631) References:

1. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.  |  Draper, JS., et al. 2002. J Anat. 200: 249-58. PMID: 12033729
2. SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers.  |  Suzuki, Y., et al. 2013. Int J Oncol. 42: 161-7. PMID: 23175153
3. Human embryonal carcinoma cells and their differentiation in culture.  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
4. Development of Fluorescently Labeled SSEA-3, SSEA-4, and Globo-H Glycosphingolipids for Elucidating Molecular Interactions in the Cell Membrane.  |  Asano, S., et al. 2019. Int J Mol Sci. 20: PMID: 31817926
5. Role of glycosphingolipid SSEA-3 and FGF2 in the stemness and lineage commitment of multilineage differentiating stress enduring (MUSE) cells.  |  Aprile, D., et al. 2023. Cell Prolif. 56: e13345. PMID: 36225120
6. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells.  |  Kannagi, R., et al. 1983. EMBO J. 2: 2355-61. PMID: 6141938
7. Immunohistochemical localization of murine stage-specific embryonic antigens in human testicular germ cell tumors.  |  Damjanov, I., et al. 1982. Am J Pathol. 108: 225-30. PMID: 6152898
8. New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3.  |  Kannagi, R., et al. 1983. J Biol Chem. 258: 8934-42. PMID: 6863318
9. Cell-surface antigens of a clonal human embryonal carcinoma cell line: morphological and antigenic differentiation in culture.  |  Andrews, PW., et al. 1982. Int J Cancer. 29: 523-31. PMID: 7095898
10. Isolation of a primate embryonic stem cell line.  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
11. Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts.  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
12. Derivation of pluripotent stem cells from cultured human primordial germ cells.  |  Shamblott, MJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 13726-31. PMID: 9811868

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SSEA-3 Antibody (631) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-SSEA-3 Antibody (631) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SSEA-3 Antibody (631) Specifications

Alternate Product Name: Stage-specific embryonic antigen 3 (SSEA3)

Product Clone Name: 631

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM

Amino Acids:

Epitope Target: SSEA-3

Epitope Species: mouse

Product Reactivity/Detection: SSEA-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, FCM

Vialing Information: Each vial contains 200 µg IgM in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for SSEA-3 Antibody (631): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin