SSEA-3 Antibody (3H420): sc-73066
- SSEA-3 Antibody (3H420) is a rat monoclonal IgM, cited in 2 publications, provided at 200 µg/ml
- raised against 4-8 cell stage mouse embryos
- recommended for detection of SSEA-3 of mouse, rat and human origin by IF and indirect
- See SSEA-3 (631): sc-21703 for SSEA-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SSEA-3 Antibody (3H420). This work is in progress.
SSEA-3 Antibody (3H420) is a rat monoclonal IgM antibody that detects SSEA-3 in mouse, rat, and human samples through immunofluorescence (IF) applications. SSEA-3, or Stage Specific Embryonic Antigen-3, plays a crucial role in identifying and characterizing undifferentiated embryonic stem cells and embryonal carcinoma cells, serving as a marker for pluripotency. This glycosylated protein is primarily located on the cell surface, where SSEA-3 is involved in cell-cell interactions and signaling pathways that maintain the undifferentiated state of stem cells. SSEA-3 expression helps distinguish between different stages of cell differentiation, providing insights into developmental biology and potential therapeutic applications in regenerative medicine. SSEA-3 interacts with other proteins involved in stem cell maintenance and differentiation, further underscoring SSEA-3′s significance in stem cell research. Anti-SSEA-3 antibody (3H420) is an essential tool for researchers studying stem cell biology, enabling the visualization and analysis of SSEA-3 expression in various cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SSEA-3 Antibody (3H420) References:
- The beta 1,3-galactosyltransferase beta 3GalT-V is a stage-specific embryonic antigen-3 (SSEA-3) synthase. | Zhou, D., et al. 2000. J Biol Chem. 275: 22631-4. PMID: 10837462
- Synthesis of cancer-associated glycoantigens: stage-specific embryonic antigen 3 (SSEA-3). | Pornsuriyasak, P. and Demchenko, AV. 2006. Carbohydr Res. 341: 1458-66. PMID: 16643871
- Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo. | Williams, SA. and Stanley, P. 2009. Glycoconj J. 26: 335-47. PMID: 18773292
- A pluripotent human stem-cell clone isolated from the TERA-2 teratocarcinoma line lacks antigens SSEA-3 and SSEA-4 in vitro, but expresses these antigens when grown as a xenograft tumor. | Andrews, PW., et al. 1985. Differentiation. 29: 127-35. PMID: 2412924
- Continuous hypoxic culturing of human embryonic stem cells enhances SSEA-3 and MYC levels. | Närvä, E., et al. 2013. PLoS One. 8: e78847. PMID: 24236059
- Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers. | Kinoshita, K., et al. 2015. Stem Cells Transl Med. 4: 146-55. PMID: 25561682
- Stage-specific embryonic antigen-3 (SSEA-3) and β3GalT5 are cancer specific and significant markers for breast cancer stem cells. | Cheung, SK., et al. 2016. Proc Natl Acad Sci U S A. 113: 960-5. PMID: 26677875
- Possibility of inhibiting arthritis and joint destruction by SSEA-3 positive cells derived from synovial tissue in rheumatoid arthritis. | Kurose, R., et al. 2017. Regen Ther. 7: 82-88. PMID: 30271856
- A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood. | Sato, T., et al. 2020. Cell Transplant. 29: 963689720923574. PMID: 32525407
- Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. | Kannagi, R., et al. 1983. EMBO J. 2: 2355-61. PMID: 6141938
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSEA-3 Antibody (3H420) | sc-73066 | 200 µg/ml | $322.00 |