SSEA-1 Antibody (480): sc-21702

SSEA-1 Antibody (480) is a mouse monoclonal IgM kappa light chain antibody that detects Stage-Specific Embryonic Antigen-1 (SSEA-1) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-SSEA-1 antibody (480) is specifically raised against X-irradiated F9 teratocarcinoma stem cells and targets SSEA-1, also known as CD15, Lewis X antigen, or sialyl Lewis X (sLeX). SSEA-1 is a carbohydrate antigen that plays a pivotal role in cell adhesion, migration, and differentiation during embryonic development, making SSEA-1 essential for proper embryogenesis and leukocyte trafficking. SSEA-1 function is particularly important as SSEA-1 mediates cell-cell interactions through binding to selectins, which are crucial for the movement of leukocytes to sites of inflammation and for the overall development of the immune system. Structurally, SSEA-1 is a tetrasaccharide carbohydrate epitope that influences various cell signaling pathways, thereby impacting cellular behavior. In murine models, SSEA-1 serves as a marker for undifferentiated embryonic stem cells, highlighting SSEA-1′s significance in stem cell research and the understanding of developmental processes. Furthermore, SSEA-1/CD15 is expressed on mature granulocytes and certain cancer cells in humans, linking SSEA-1 to immune responses and cancer metastasis. SSEA-1 (480) monoclonal antibody facilitates the exploration of stem cell biology, cell differentiation pathways, and the mechanisms underlying various diseases.

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SSEA-1 Antibody (480) References:

1. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.  |  Draper, JS., et al. 2002. J Anat. 200: 249-58. PMID: 12033729
2. Human embryonal carcinoma cells and their differentiation in culture.  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
3. Stage-Specific Embryonic Antigen-1 (SSEA-1) Expression in Thyroid Tissues.  |  Xu, J., et al. 2016. Endocr Pathol. 27: 271-275. PMID: 27550342
4. Selectin Ligands Sialyl-Lewis a and Sialyl-Lewis x in Gastrointestinal Cancers.  |  Trinchera, M., et al. 2017. Biology (Basel). 6: PMID: 28241499
5. Bone Morphogenetic Protein Promotes Lewis X Stage-Specific Embryonic Antigen 1 Expression Thereby Interfering with Neural Precursor and Stem Cell Proliferation.  |  Luque-Molina, I., et al. 2017. Stem Cells. 35: 2417-2429. PMID: 28869691
6. Prognostic and Therapeutic Role of CD15 and CD15s in Cancer.  |  Szlasa, W., et al. 2022. Cancers (Basel). 14: PMID: 35565333
7. The Sialyl Lewis X Glycan Receptor Facilitates Infection of Subtype H7 Avian Influenza A Viruses.  |  Guan, M., et al. 2022. J Virol. 96: e0134422. PMID: 36125302
8. Sialyl LewisX/A and Cytokeratin Crosstalk in Triple Negative Breast Cancer.  |  Pascoal, C., et al. 2023. Cancers (Basel). 15: PMID: 36765690
9. SSEA-1 Correlates With the Invasive Phenotype in Breast Cancer.  |  Kohler, KT., et al. 2023. J Histochem Cytochem. 71: 423-430. PMID: 37477396
10. Isolation of a primate embryonic stem cell line.  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
11. Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts.  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
12. Derivation of pluripotent stem cells from cultured human primordial germ cells.  |  Shamblott, MJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 13726-31. PMID: 9811868

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SSEA-1 Antibody (480) has 187 citations in a variety of scientific publications.

Primary antibodies like Anti-SSEA-1 Antibody (480) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SSEA-1 Antibody (480) Specifications

Alternate Product Name: Stage-specific embryonic antigen 1 (SSEA1)

Product Clone Name: 480

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: SSEA-1

Epitope Species: human

Product Reactivity/Detection: SSEA-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 187 citations in various scientific publications

Available Conjugates/Forms for SSEA-1 Antibody (480): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin