ss/ds DNA marker Antibody (3H12): sc-73065

ss/ds DNA marker Antibody (3H12) is a mouse monoclonal IgG2b antibody that detects the ss/ds DNA marker protein of mammalian origin by western blotting (WB). Anti-ss/ds DNA marker antibody (3H12) is available as the non-conjugated format. Deoxyribonucleic acid (DNA) plays a crucial role in storing and transmitting genetic information, serving as the blueprint for the development and function of all known living organisms. The structure of DNA, which typically exists as a double-stranded molecule forming a double helix, is vital for stability and function. This helical structure allows for specific pairing of nucleotide bases—adenine with thymine and guanine with cytosine—ensuring accurate replication and transcription processes. When DNA unwinds, single-stranded forms become essential for various cellular processes, including replication and transcription, as proteins can access the genetic code. ss/ds DNA marker (3H12) antibody detects both single-stranded and double-stranded DNA in various samples, making ss/ds DNA marker (3H12) monoclonal antibody an invaluable tool for studying genetic material and its functions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ss/ds DNA marker Antibody (3H12) References:

1. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid.  |  WATSON, JD. and CRICK, FH. 1953. Nature. 171: 737-8. PMID: 13054692
2. Unraveling DNA helicases. Motif, structure, mechanism and function.  |  Tuteja, N. and Tuteja, R. 2004. Eur J Biochem. 271: 1849-63. PMID: 15128295
3. Single-stranded adenine-rich DNA and RNA retain structural characteristics of their respective double-stranded conformations and show directional differences in stacking pattern.  |  Isaksson, J., et al. 2004. Biochemistry. 43: 15996-6010. PMID: 15609994
4. DNA mechanics.  |  Benham, CJ. and Mielke, SP. 2005. Annu Rev Biomed Eng. 7: 21-53. PMID: 16004565
5. Computational structural analysis: multiple proteins bound to DNA.  |  Tomovic, A. and Oakeley, EJ. 2008. PLoS One. 3: e3243. PMID: 18802470
6. Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors.  |  Paix, A., et al. 2019. Curr Protoc Mol Biol. 129: e102. PMID: 31710422
7. Unambiguous Discrimination of Multiple Protein Biomarkers by Nanopore Sensing with Double-Stranded DNA-Based Probes.  |  Fang, Z., et al. 2020. Anal Chem. 92: 1730-1737. PMID: 31869203
8. Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells.  |  Ayares, D., et al. 1987. Mol Cell Biol. 7: 1656-62. PMID: 3474515
9. Studies on the functions of DNA helicase I and DNA helicase II of Escherichia coli.  |  Klinkert, MQ., et al. 1980. J Biol Chem. 255: 9746-52. PMID: 6107294
10. Protein-DNA recognition.  |  Pabo, CO. and Sauer, RT. 1984. Annu Rev Biochem. 53: 293-321. PMID: 6236744
11. Open complex formation by Escherichia coli RNA polymerase: the mechanism of polymerase-induced strand separation of double helical DNA.  |  deHaseth, PL. and Helmann, JD. 1995. Mol Microbiol. 16: 817-24. PMID: 7476180
12. Instability and decay of the primary structure of DNA.  |  Lindahl, T. 1993. Nature. 362: 709-15. PMID: 8469282

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ss/ds DNA marker Antibody (3H12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ss/ds DNA marker Antibody (3H12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ss/ds DNA marker Antibody (3H12) Specifications

Alternate Product Name: ss/ds DNA marker

Product Clone Name: 3H12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: ss/ds DNA marker

Epitope Species: bovine

Product Reactivity/Detection: single or double stranded eukaryotic DNA

Species Reactivity/Detection: mammalian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ss/ds DNA marker Antibody (3H12): N/A

The preferred ds DNA Marker antibody is ds DNA Marker Antibody (HYB331-01): sc-58749.