SRPK2 Antibody (H-5): sc-390534
- SRPK2 Antibody (H-5) is a mouse monoclonal IgG1 κ SRPK2 antibody provided at 200 µg/ml
- raised against amino acids 372-452 mapping within an internal region of SRPK2 of human origin
- SRPK2 Antibody (H-5) is recommended for detection of SRPK2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SRPK2 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SRPK2 Antibody (H-5) for WB applications. These reagents are now offered in bundles with SRPK2 Antibody (H-5) (see ordering information below).
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SRPK2 Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects SRPK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRPK2 (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SRPK2 protein plays a crucial role in the regulation of pre-mRNA splicing, a vital process for gene expression, by phosphorylating arginine/serine-rich (RS) domain-containing proteins. This phosphorylation is essential for the proper functioning of splicing factors, which coordinate splicing with transcription, ensuring that mRNA is accurately processed before translation. Notably, SRPK2 exhibits a stringent preference for SR dipeptides and features a proline-rich sequence at its amino terminus, which may influence its interaction with other proteins involved in splicing regulation. The expression of SRPK2 in various tissues, including the brain, heart, liver, and lung, highlights its importance in diverse cellular processes, making anti-SRPK2 antibody (H-5) a valuable tool for researchers studying gene expression and splicing mechanisms in different biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SRPK2 Antibody (H-5) References:
- Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. | Wang, HY., et al. 1999. Genomics. 57: 310-5. PMID: 10198174
- SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1. | Papoutsopoulou, S., et al. 1999. Nucleic Acids Res. 27: 2972-80. PMID: 10390541
- Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1. | Tang, Z., et al. 2000. Mol Cell Biol. 20: 816-24. PMID: 10629038
- Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons. | Jang, SW., et al. 2009. J Biol Chem. 284: 24512-25. PMID: 19592491
- The N-terminal fragment from caspase-cleaved serine/arginine protein-specific kinase2 (SRPK2) translocates into the nucleus and promotes apoptosis. | Hong, Y., et al. 2011. J Biol Chem. 286: 777-86. PMID: 21056976
- Primary structural features of SR-like protein acinusS govern the phosphorylation mechanism by SRPK2. | Liang, N., et al. 2014. Biochem J. 459: 181-91. PMID: 24444330
- Transcription Dynamics Prevent RNA-Mediated Genomic Instability through SRPK2-Dependent DDX23 Phosphorylation. | Sridhara, SC., et al. 2017. Cell Rep. 18: 334-343. PMID: 28076779
- Enhanced expression of SRPK2 contributes to aggressive progression and metastasis in prostate cancer. | Zhuo, YJ., et al. 2018. Biomed Pharmacother. 102: 531-538. PMID: 29587239
- CRISPR/Cas9-mediated double knockout of SRPK1 and SRPK2 in a nasopharyngeal carcinoma cell line. | Prattapong, P., et al. 2020. Cancer Rep (Hoboken). 3: e1224. PMID: 32671994
- Impaired expression of serine/arginine protein kinase 2 (SRPK2) affects melanoma progression. | Caetano, MMM., et al. 2022. Front Genet. 13: 979735. PMID: 36212152
- Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles. | Kuroyanagi, N., et al. 1998. Biochem Biophys Res Commun. 242: 357-64. PMID: 9446799
- SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. | Wang, HY., et al. 1998. J Cell Biol. 140: 737-50. PMID: 9472028
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRPK2 Antibody (H-5) | sc-390534 | 200 µg/ml | $322.00 | |||
SRPK2 Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-523572 | 200 µg Ab, 40 µg BP | $361.00 | |||
SRPK2 Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-533593 | 200 µg Ab; 20 µg BP | $361.00 | |||
SRPK2 Antibody (H-5) AC | sc-390534 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SRPK2 Antibody (H-5) HRP | sc-390534 HRP | 200 µg/ml | $322.00 | |||
SRPK2 Antibody (H-5) FITC | sc-390534 FITC | 200 µg/ml | $336.00 | |||
SRPK2 Antibody (H-5) PE | sc-390534 PE | 200 µg/ml | $349.00 | |||
SRPK2 Antibody (H-5) Alexa Fluor® 488 | sc-390534 AF488 | 200 µg/ml | $364.00 | |||
SRPK2 Antibody (H-5) Alexa Fluor® 546 | sc-390534 AF546 | 200 µg/ml | $364.00 | |||
SRPK2 Antibody (H-5) Alexa Fluor® 594 | sc-390534 AF594 | 200 µg/ml | $364.00 | |||
SRPK2 Antibody (H-5) Alexa Fluor® 647 | sc-390534 AF647 | 200 µg/ml | $364.00 | |||
SRPK2 Antibody (H-5) Alexa Fluor® 680 | sc-390534 AF680 | 200 µg/ml | $364.00 | |||
SRPK2 Antibody (H-5) Alexa Fluor® 790 | sc-390534 AF790 | 200 µg/ml | $364.00 |