Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SREBP-1 CRISPR/Cas9 KO Plasmid (m): sc-423152
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SREBP-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SREBP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SREBP-1 Antibody (A-4): sc-365513
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SREBP-1 CRISPR/Cas9 KO Plasmid (m) | sc-423152 | 20 µg | $397.00 |
Overview
Srebf1 encodes sterol regulatory element-binding protein 1 (SREBP-1), a membrane-tethered transcription factor that is proteolytically activated to control lipid and carbohydrate metabolism programs. In mouse cells, SREBP-1 drives expression of enzymes required for de novo fatty acid and triglyceride synthesis and links nutrient and hormonal cues to lipogenic gene networks through SREBP-dependent transcriptional regulation. This pathway integrates with insulin and mTOR signaling, ER homeostasis, and membrane biogenesis, influencing adipogenesis and hepatocyte metabolic remodeling. Dysregulated SREBP-1 activity is widely used as a mechanistic axis in models of hepatic steatosis, obesity-associated insulin resistance, and lipid-driven inflammation.
SREBP-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Srebf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Srebf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Srebf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SREBP-1 protein expression.
This CRISPR knockout system enables efficient generation of Srebf1-deficient cell models for investigation of SREBP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Srebf1 exon(s) critical for SREBP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Srebf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SREBP-1 CRISPR/Cas9 KO Plasmid (m) and SREBP-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Srebf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SREBP-1 HDR Plasmid (m) and SREBP-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Srebf1 homology arms to support homology-directed repair at defined Srebf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.