SRA Antibody (C-5): sc-271377

SRA Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects steroid receptor RNA activator (SRA) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRA plays a crucial role in mediating the transactivation of steroid hormone receptors, functioning as both an RNA transcript and a stably expressed protein. This dual nature allows SRA to form complexes with steroid receptor coactivator-1, enhancing the transcriptional activity of estrogen receptor alpha (ERalpha). Notably, SRA contains six essential RNA motifs that are integral to its coactivation capabilities. SRA is widely expressed across various normal tissues, with particularly high levels found in the liver and skeletal muscle, while exhibiting lower expression in the brain. SRA is overexpressed in breast tumors compared to normal tissue, suggesting a potential role in cancer biology. Furthermore, the interaction between SRA and the SMRT/HDAC1 associated repressor protein (SHARP) highlights a regulatory mechanism where SHARP can inhibit SRA-potentiated steroid receptor transcription, underscoring the importance of SRA in hormonal signaling pathways. anti-SRA antibody (C-5) is an invaluable tool for researchers investigating the complexities of steroid receptor regulation and its implications in health and disease.

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SRA Antibody (C-5) References:

1. Steroid Receptor RNA Activator Protein (SRAP): a potential new prognostic marker for estrogen receptor-positive/node-negative/younger breast cancer patients.  |  Yan, Y., et al. 2009. Breast Cancer Res. 11: R67. PMID: 19740422
2. The steroid receptor RNA activator protein is recruited to promoter regions and acts as a transcriptional repressor.  |  Chooniedass-Kothari, S., et al. 2010. FEBS Lett. 584: 2218-24. PMID: 20398657
3. Steroid receptor RNA activator protein binds to and counteracts SRA RNA-mediated activation of MyoD and muscle differentiation.  |  Hubé, F., et al. 2011. Nucleic Acids Res. 39: 513-25. PMID: 20855289
4. Structure and function of steroid receptor RNA activator protein, the proposed partner of SRA noncoding RNA.  |  McKay, DB., et al. 2014. J Mol Biol. 426: 1766-1785. PMID: 24486609
5. Increased steroid receptor RNA activator protein (SRAP) accompanied by decreased estrogen receptor-beta (ER-β) levels during the malignant transformation of endometriosis associated ovarian clear cell carcinoma.  |  Lin, K., et al. 2014. Acta Histochem. 116: 878-82. PMID: 24704270
6. Steroid receptor RNA activator affects the development of poststroke depression by regulating the peroxisome proliferator-activated receptor γ signaling pathway.  |  Jiang, B., et al. 2020. Neuroreport. 31: 48-56. PMID: 31714481
7. Steroid Receptor RNA Activator, a Long Noncoding RNA, Activates p38, Facilitates Epithelial-Mesenchymal Transformation, and Mediates Experimental Melanoma Metastasis.  |  Hong, CH., et al. 2020. J Invest Dermatol. 140: 1355-1363.e1. PMID: 31945347
8. Targeting steroid receptor RNA activator (SRA), a long non-coding RNA, enhances melanogenesis through activation of TRP1 and inhibition of p38 phosphorylation.  |  Ho, JC., et al. 2020. PLoS One. 15: e0237577. PMID: 32790741
9. Steroid receptor RNA activator inhibits the migration, invasion and stemness characteristics of renal cell carcinoma cells.  |  Zhang, C., et al. 2020. Int J Mol Med. 46: 1765-1776. PMID: 33000206
10. Long noncoding RNA steroid receptor RNA activator 1 inhibits proliferation and glycolysis of esophageal squamous cell carcinoma.  |  He, M., et al. 2024. World J Gastrointest Oncol. 16: 4194-4208. PMID: 39473951

The preferred SRA antibody is SRA Antibody (E-5): sc-393240.