SR Antibody (1H4): sc-13509

SR Antibody (1H4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). SR (1H4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. SR protein family plays a crucial role in pre-mRNA splicing, which is essential for accurate processing of mRNA and regulation of gene expression. SR proteins are primarily located in the nucleus, where SR proteins bind to specific RNA sequences known as splicing enhancer elements, which are vital for recognition of splice sites and control of alternative splicing. Presence of serine/arginine-rich (SR) splicing factors, such as SC35 and SRp40, is critical for constitutive splicing, while other members like SF2/ASF and SRp20 shuttle between nucleus and cytoplasm, facilitating mRNA transport. Intricate balance of SR proteins and interactions with other splicing factors, including members of heterogeneous nuclear ribonucleoprotein (hnRNP) family, underscores importance in regulation of splicing events, making SR (1H4) monoclonal antibody an invaluable tool for researchers studying gene expression and splicing mechanisms in human cells.

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SR Antibody (1H4) References:

1. The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation.  |  Petersen-Mahrt, SK., et al. 1999. EMBO J. 18: 1014-24. PMID: 10022843
2. Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.  |  Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
3. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.  |  Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
4. Physiological and Pathological Function of Serine/Arginine-Rich Splicing Factor 4 and Related Diseases.  |  Tan, W., et al. 2018. Biomed Res Int. 2018: 3819719. PMID: 29789787
5. Noncanonical functions of the serine-arginine-rich splicing factor (SR) family of proteins in development and disease.  |  Wagner, RE. and Frye, M. 2021. Bioessays. 43: e2000242. PMID: 33554347
6. Serine arginine-rich splicing factor (SRSF7) cooperates with the histone methyltransferase KMT5a to promote the type I interferon response via transcriptional activation of IRF7.  |  Scott, HM., et al. 2023. bioRxiv.. PMID: 37503164
7. Targeting alternative splicing as a new cancer immunotherapy-phosphorylation of serine arginine-rich splicing factor (SRSF1) by SR protein kinase 1 (SRPK1) regulates alternative splicing of PD1 to generate a soluble antagonistic isoform that prevents T cell exhaustion.  |  Wahid, M., et al. 2023. Cancer Immunol Immunother. 72: 4001-4014. PMID: 37973660
8. Serine/arginine-rich splicing factor 7 promotes the type I interferon response by activating Irf7 transcription.  |  Scott, HM., et al. 2024. Cell Rep. 43: 113816. PMID: 38393946
9. Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins.  |  Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
10. Specific commitment of different pre-mRNAs to splicing by single SR proteins.  |  Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
11. The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation.  |  Jumaa, H. and Nielsen, PJ. 1997. EMBO J. 16: 5077-85. PMID: 9305649
12. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm.  |  Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SR Antibody (1H4) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-SR Antibody (1H4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SR Antibody (1H4) Specifications

Alternate Product Name: Serine And Arginine Rich Splicing Factor

Product Clone Name: 1H4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SR

Epitope Species: Xenopus

Product Reactivity/Detection: SR RNA processing factors, including SRp75, SRp55, SRp40, SRp30a/b and SRp20

Species Reactivity/Detection: human

Additional Mammalian Reactivity: bovine and avian

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for SR Antibody (1H4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin