SR-3A Double Nickase Plasmid (h): sc-402999-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SR-3A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SR-3A Double Nickase Plasmid (h) and SR-3A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HTR3A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SR-3A Antibody (A-9): sc-390168
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SR-3A Double Nickase Plasmid (h) | sc-402999-NIC | 20 µg | $410.00 | |||
SR-3A Double Nickase Plasmid (h2) | sc-402999-NIC-2 | 20 µg | $410.00 |
HTR3A encodes the human 5-hydroxytryptamine (serotonin) receptor 3A (SR-3A), a ligand-gated ion channel of the Cys-loop receptor family that forms homopentameric or heteropentameric 5-HT3 receptors with other subunits. Upon serotonin binding, SR-3A mediates rapid cation influx to regulate membrane depolarization, calcium-dependent signaling, and neurotransmitter release, linking serotonergic tone to synaptic transmission and neuroimmune communication. HTR3A activity intersects with pathways controlling neuronal excitability and downstream MAPK/ERK and cAMP-responsive transcriptional programs through changes in ionic homeostasis. Dysregulated 5-HT3 signaling has been associated with neuropsychiatric and gastrointestinal phenotypes, supporting investigation of HTR3A in brain–gut axis biology and stimulus-evoked cellular responses.
SR-3A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HTR3A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HTR3A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HTR3A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HTR3A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.