SR-2C CRISPR/Cas9 KO Plasmid (h): sc-400886

Overview

HTR2C encodes the human serotonin 2C receptor (SR-2C), a G protein–coupled receptor that primarily couples to Gq/11 to stimulate phospholipase C signaling, inositol trisphosphate production, intracellular Ca2+ mobilization, and protein kinase C activation. SR-2C also engages MAPK/ERK pathways and modulates neuronal excitability and synaptic transmission through effects on ion channel activity and second-messenger dynamics. In the central nervous system, HTR2C contributes to regulation of appetite, mood, stress responsivity, and endocrine outputs, integrating serotonergic inputs with downstream transcriptional and metabolic programs. Dysregulation of 5-HT2C signaling and RNA editing/splice variation within HTR2C has been associated in research literature with neuropsychiatric and metabolic phenotypes, supporting its utility as a mechanistic target for pathway and genotype–phenotype studies.

SR-2C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HTR2C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HTR2C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HTR2C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SR-2C protein expression.

This CRISPR knockout system enables efficient generation of HTR2C-deficient cell models for investigation of SR-2C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting HTR2C exon(s) critical for SR-2C function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple HTR2C genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by SR-2C CRISPR/Cas9 KO Plasmid (h) and SR-2C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HTR2C locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by SR-2C HDR Plasmid (h) and SR-2C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HTR2C homology arms to support homology-directed repair at defined HTR2C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.