SR-2B Double Nickase Plasmid (m): sc-420994-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SR-2B Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SR-2B Double Nickase Plasmid (m) and SR-2B Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Htr2b. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SR-2B Antibody (C-6): sc-376878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SR-2B Double Nickase Plasmid (m) | sc-420994-NIC | 20 µg | $410.00 | |||
SR-2B Double Nickase Plasmid (m2) | sc-420994-NIC-2 | 20 µg | $410.00 |
Mouse Htr2b encodes the serotonin receptor SR-2B (5-HT2B), a G protein–coupled receptor that primarily couples to Gq/11 to stimulate phospholipase C signaling, inositol phosphate production, intracellular Ca2+ mobilization, and downstream PKC/MAPK pathway activity. SR-2B contributes to serotonergic regulation of vascular tone, cardiac development and remodeling, and CNS processes including synaptic signaling and neuroinflammatory responses. Altered Htr2b activity has been implicated in pathways relevant to cardiovascular dysfunction and fibrotic remodeling, and in neuropsychiatric phenotypes linked to serotonergic circuit imbalance. These features make Htr2b a useful target for dissecting GPCR-driven second-messenger signaling, transcriptional responses, and tissue-specific serotonergic regulation in mouse models.
SR-2B Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Htr2b locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Htr2b. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Htr2b function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Htr2b-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.