SR-2B Double Nickase Plasmid (h): sc-402574-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SR-2B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SR-2B Double Nickase Plasmid (h) and SR-2B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HTR2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SR-2B Antibody (C-6): sc-376878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SR-2B Double Nickase Plasmid (h) | sc-402574-NIC | 20 µg | $410.00 | |||
SR-2B Double Nickase Plasmid (h2) | sc-402574-NIC-2 | 20 µg | $410.00 |
HTR2B encodes the human 5-hydroxytryptamine receptor 2B (SR-2B), a G protein–coupled receptor that primarily couples to Gq/11 to stimulate phospholipase C signaling, inositol phosphate turnover, intracellular Ca²⁺ mobilization, and downstream PKC/MAPK pathway activation. SR-2B can also engage β-arrestin–dependent signaling and receptor desensitization, linking serotonergic cues to transcriptional programs that regulate cell proliferation, migration, and extracellular matrix remodeling. HTR2B activity is relevant to neurovascular biology and peripheral serotonergic signaling, with reported associations to cardiopulmonary and neuropsychiatric phenotypes in genetic and functional studies. In biomedical research, HTR2B is frequently investigated as a modulator of receptor cross-talk and GPCR network behavior in systems where serotonin shapes developmental, inflammatory, or tissue remodeling responses.
SR-2B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HTR2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HTR2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HTR2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HTR2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.