Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SR-2A CRISPR/Cas9 KO Plasmid (h): sc-401532
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SR-2A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SR-2A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SR-2A Antibody (A-4): sc-166775
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SR-2A CRISPR/Cas9 KO Plasmid (h) | sc-401532 | 20 µg | $397.00 |
Overview
HTR2A encodes the human 5-hydroxytryptamine receptor 2A (SR-2A), a G protein–coupled receptor that primarily couples to Gq/11 to stimulate phospholipase C signaling, inositol phosphate turnover, intracellular Ca²⁺ mobilization, and protein kinase C activation. SR-2A modulates neuronal excitability and synaptic plasticity and interfaces with broader neurotransmitter networks through cross-talk with MAPK/ERK and related second-messenger pathways. Expression and signaling changes in HTR2A are frequently investigated in the context of neuropsychiatric phenotypes, including altered serotonergic tone and receptor-mediated cortical circuit function. As a membrane receptor with complex regulatory dynamics, SR-2A provides a tractable node for dissecting pathway-specific signaling and receptor desensitization/internalization mechanisms in human cellular models.
SR-2A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HTR2A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HTR2A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HTR2A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SR-2A protein expression.
This CRISPR knockout system enables efficient generation of HTR2A-deficient cell models for investigation of SR-2A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HTR2A exon(s) critical for SR-2A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HTR2A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SR-2A CRISPR/Cas9 KO Plasmid (h) and SR-2A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HTR2A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SR-2A HDR Plasmid (h) and SR-2A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HTR2A homology arms to support homology-directed repair at defined HTR2A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.