Anti-Squalene epoxidase Antibody (H-6) recommended for detection of Squalene epoxidase of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Squalene epoxidase Antibody (H-6): sc-271651

Anti-Squalene epoxidase Antibody (H-6) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-Squalene epoxidase Antibody (H-6) is a mouse monoclonal IgG2b κ Squalene epoxidase antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against amino acids 275-574 mapping at the C-terminus of Squalene epoxidase of human origin
    • Squalene epoxidase Antibody (H-6) is recommended for detection of Squalene epoxidase of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Squalene epoxidase Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Squalene epoxidase (H-6): sc-271651.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Squalene epoxidase Antibody (H-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with Squalene epoxidase Antibody (H-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I am using Squalene epoxidase (H-6): sc-271651 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good Antibody works fine, good signals in western blot data !!
Date published: 2018-02-07
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of Squalene epoxidase expression in Caki-1 whole cell lysate.
Date published: 2017-01-23
Rated 5 out of 5 by from Beautiful immunofluorescence cytoplasmic Beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-17
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