Squalene epoxidase Antibody (H-6): sc-271651

Squalene epoxidase Antibody (H-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects the Squalene epoxidase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Squalene epoxidase antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Squalene epoxidase, also known as Squalene monooxygenase, plays a crucial role in the cholesterol biosynthetic pathway by catalyzing the first oxygenation step, which is essential for the conversion of squalene to 2,3-oxidosqualene. This reaction is a rate-limiting step in sterol biosynthesis, making Squalene epoxidase vital for maintaining cellular cholesterol levels, which are critical for membrane integrity, hormone synthesis, and overall cellular function. Squalene epoxidase is located in the endoplasmic reticulum and interacts with Squalene synthetase, suggesting a coordinated regulation of cholesterol biosynthesis. The ability of Squalene epoxidase to form complexes with other proteins in the pathway highlights its importance in cellular lipid metabolism and potential implications in diseases related to cholesterol dysregulation.

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Squalene epoxidase Antibody (H-6) References:

1. Squalene epoxidase encoded by ERG1 affects morphogenesis and drug susceptibilities of Candida albicans.  |  Pasrija, R., et al. 2005. J Antimicrob Chemother. 55: 905-13. PMID: 15845783
2. Inhibition of cholesterol biosynthesis by 25-hydroxycholesterol is independent of OSBP.  |  Nishimura, T., et al. 2005. Genes Cells. 10: 793-801. PMID: 16098143
3. Characterizing sterol defect suppressors uncovers a novel transcriptional signaling pathway regulating zymosterol biosynthesis.  |  Germann, M., et al. 2005. J Biol Chem. 280: 35904-13. PMID: 16120615
4. Studies of the FABP family: a retrospective.  |  Ono, T. 2005. Mol Cell Biochem. 277: 1-6. PMID: 16132708
5. Dual roles for cholesterol in mammalian cells.  |  Xu, F., et al. 2005. Proc Natl Acad Sci U S A. 102: 14551-6. PMID: 16199524
6. Single amino acid exchanges in FAD-binding domains of squalene epoxidase of Saccharomyces cerevisiae lead to either loss of functionality or terbinafine sensitivity.  |  Ruckenstuhl, C., et al. 2005. Biochem Soc Trans. 33: 1197-201. PMID: 16246080
7. Squalene epoxidase drives NAFLD-induced hepatocellular carcinoma and is a pharmaceutical target.  |  Liu, D., et al. 2018. Sci Transl Med. 10: PMID: 29669855
8. Squalene Epoxidase Induces Nonalcoholic Steatohepatitis Via Binding to Carbonic Anhydrase III and is a Therapeutic Target.  |  Liu, D., et al. 2021. Gastroenterology. 160: 2467-2482.e3. PMID: 33647280
9. Squalene epoxidase drives cancer cell proliferation and promotes gut dysbiosis to accelerate colorectal carcinogenesis.  |  Li, C., et al. 2022. Gut. 71: 2253-2265. PMID: 35232776
10. Identifying squalene epoxidase as a metabolic vulnerability in high-risk osteosarcoma using an artificial intelligence-derived prognostic index.  |  Wang, Y., et al. 2024. Clin Transl Med. 14: e1586. PMID: 38372422
11. Targeting squalene epoxidase restores anti-PD-1 efficacy in metabolic dysfunction-associated steatohepatitis-induced hepatocellular carcinoma.  |  Wen, J., et al. 2024. Gut. 73: 2023-2036. PMID: 38744443
12. Localization of the squalene epoxidase gene (SQLE) to human chromosome region 8q24.1.  |  Nagai, M., et al. 1997. Genomics. 44: 141-3. PMID: 9286711

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Squalene epoxidase Antibody (H-6) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Squalene epoxidase Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Squalene epoxidase Antibody (H-6) Specifications

Alternate Product Name: Squalene epoxidase

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 275-574

Epitope Target: Squalene epoxidase

Epitope Species: human

Product Reactivity/Detection: Squalene epoxidase

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Squalene epoxidase Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin