SPUVE CRISPR/Cas9 KO Plasmid (h): sc-406568
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPUVE CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPUVE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPUVE CRISPR/Cas9 KO Plasmid (h) | sc-406568 | 20 µg | $397.00 |
Overview
PRSS23 encodes the serine protease SPUVE, a trypsin-like enzyme implicated in pericellular proteolysis and remodeling of the extracellular milieu. SPUVE activity has been linked to regulation of epithelial and stromal cell behavior, including migration, adhesion, and differentiation programs that intersect with protease-activated signaling and extracellular matrix dynamics. Expression and functional studies associate PRSS23 with hormone-responsive and inflammatory contexts, suggesting relevance to tissue remodeling and dysregulated growth phenotypes. These properties make PRSS23 a useful target for dissecting protease-dependent pathways that influence cell state transitions and microenvironmental interactions.
SPUVE CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRSS23 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRSS23 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRSS23 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPUVE protein expression.
This CRISPR knockout system enables efficient generation of PRSS23-deficient cell models for investigation of SPUVE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRSS23 exon(s) critical for SPUVE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRSS23 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPUVE CRISPR/Cas9 KO Plasmid (h) and SPUVE CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRSS23 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPUVE HDR Plasmid (h) and SPUVE HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRSS23 homology arms to support homology-directed repair at defined PRSS23 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.