Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SPTLC1 Double Nickase Plasmid (m): sc-435265-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPTLC1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SPTLC1 Double Nickase Plasmid (m) and SPTLC1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sptlc1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPTLC1 Antibody (H-1): sc-374143
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPTLC1 Double Nickase Plasmid (m) | sc-435265-NIC | 20 µg | $410.00 | |||
SPTLC1 Double Nickase Plasmid (m2) | sc-435265-NIC-2 | 20 µg | $410.00 |
Sptlc1 encodes serine palmitoyltransferase long chain base subunit 1 (SPTLC1), a core component of the serine palmitoyltransferase complex that catalyzes the first and rate-limiting step of de novo sphingolipid biosynthesis. By controlling production of sphingoid bases and downstream ceramides and sphingomyelins, SPTLC1 helps regulate membrane composition, lipid signaling, and organelle function, including ER homeostasis. Altered SPTLC1 activity can perturb sphingolipid flux, impacting processes such as stress responses, mitochondrial physiology, and neuronal maintenance. SPTLC1 dysregulation is implicated in disorders linked to sphingolipid imbalance, supporting its use in mechanistic studies of lipid metabolism and neurobiology.
SPTLC1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sptlc1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sptlc1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sptlc1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sptlc1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.