Spt3 Antibody (D-11): sc-390356

Spt3 Antibody (D-11) is a mouse monoclonal IgM kappa antibody that detects Spt3 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Spt3 antibody (D-11) is available as a non-conjugated antibody, specifically designed to facilitate the study of Spt3 protein, which plays a crucial role in the transcriptional regulation of RNA polymerase II-dependent genes. Located within the SAGA complex, Spt3 is essential for the recruitment of TATA-box binding protein (TBP) to core promoters, a process vital for initiating transcription. The interaction between Spt3 and TBP is particularly important for the transcription of delta sequences, which are long terminal repeats flanking yeast Ty elements. Notably, Spt3 also exhibits a repressive effect on the binding of TBP at certain gene promoters, such as HO, HIS3, and TRP3, thereby modulating transcription levels. This repression can be counteracted by GCN5, another member of the SAGA complex, which enhances TBP/TFIIA complex formation through histone acetylation. Furthermore, null mutations in the Spt3 gene have been linked to significant defects in sporulation and various growth pathways in yeast, underscoring Spt3′s importance in both mating and developmental processes. With diverse applications, Spt3 (D-11) antibody is an invaluable tool for researchers investigating the intricate mechanisms of gene regulation in yeast.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Spt3 Antibody (D-11) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Spt3 Antibody (D-11) Specifications

Alternate Product Name: Spt3

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 50-66

Epitope Target: Spt3

Epitope Species: Saccharomyces cerevisiae

Product Reactivity/Detection: Spt3

Species Reactivity/Detection: y

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Spt3 Antibody (D-11): P: Blocking Peptide