SPT3 Antibody (71-S): sc-101157
- SPT3 Antibody (71-S) is a mouse monoclonal IgM, cited in 2 publications, provided at 200 µl ascites
- raised against recombinant SPT3 of human origin
- recommended for detection of SPT3 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SPT3 Antibody (71-S). This work is in progress.
SPT3 Antibody (71-S) is a mouse monoclonal IgM antibody that detects SPT3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). SPT3 (71-S) antibody is available as the non-conjugated form. SPT3 protein plays a crucial role in the Saccharomyces cerevisiae SAGA complex, a multifunctional coactivator that regulates transcription by RNA polymerase II. Within yeast, SPT3 is integral to the SPT-ADA-GCN5 acetyltransferase (SAGA) complex, which is essential for integrating proteins that possess transcription coactivator and adaptor functions, as well as histone acetyltransferase activity. This complex is vital for core promoter-selective functions, particularly through interactions with the TATA-binding protein. In humans, the STAGA complex, which contains homologs of most yeast SAGA components, exhibits acetyl coenzyme A-dependent transcriptional coactivator functions from chromatin-assembled templates in vitro and associates with spliceosome-associated proteins in HeLa cells. Notably, amino acid sequence comparisons between human SPT3 and yeast counterparts reveal three highly conserved domains, with the most conserved 92-amino acid N-terminal domain showing 25% identity with human TAFII18, underscoring the evolutionary significance and functional conservation of SPT3 in transcriptional regulation.
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SPT3 Antibody (71-S) References:
- Inhibition of TATA-binding protein function by SAGA subunits Spt3 and Spt8 at Gcn4-activated promoters. | Belotserkovskaya, R., et al. 2000. Mol Cell Biol. 20: 634-47. PMID: 10611242
- Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. | Martinez, E., et al. 2001. Mol Cell Biol. 21: 6782-95. PMID: 11564863
- Spt3 and Mot1 cooperate in nucleosome remodeling independently of TBP recruitment. | Topalidou, I., et al. 2004. EMBO J. 23: 1943-8. PMID: 15057269
- Molecular architecture of the S. cerevisiae SAGA complex. | Wu, PY., et al. 2004. Mol Cell. 15: 199-208. PMID: 15260971
- The NOT, SPT3, and MOT1 genes functionally interact to regulate transcription at core promoters. | Collart, MA. 1996. Mol Cell Biol. 16: 6668-76. PMID: 8943321
- Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family. | Birck, C., et al. 1998. Cell. 94: 239-49. PMID: 9695952
- A human SPT3-TAFII31-GCN5-L acetylase complex distinct from transcription factor IID. | Martinez, E., et al. 1998. J Biol Chem. 273: 23781-5. PMID: 9726987
- Characterization of a human homologue of the Saccharomyces cerevisiae transcription factor spt3 (SUPT3H). | Yu, J., et al. 1998. Genomics. 53: 90-6. PMID: 9787080
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPT3 Antibody (71-S) | sc-101157 | 200 µl ascites | $339.00 |