SPT16 Antibody (H-4): sc-165989
- SPT16 Antibody (H-4) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 748-1047 mapping at the C-terminus of SPT16 of human origin
- recommended for detection of SPT16 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
- m-IgG2b BP-HRP is the preferred secondary detection reagent for SPT16 Antibody (H-4) for WB applications. This reagent is now offered in a bundle with SPT16 Antibody (H-4) (see ordering information below).
SPT16 Antibody (H-4) is a mouse monoclonal IgG2b antibody that detects SPT16 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPT16 is a crucial component of the FACT complex, which plays a significant role in the transcription of chromatin templates by facilitating nucleosome disassembly during transcription elongation. This process is vital for gene expression regulation, as RNA polymerase can access the DNA wrapped around nucleosomes. The FACT complex, consisting of SPT16 and the structure-specific recognition protein-1 (SSRP-1), interacts specifically with histone H2A/H2B dimers, ensuring that transcription can proceed efficiently. SPT16′s ability to modulate chromatin structure is essential for proper gene expression, as SPT16 helps maintain the balance between transcriptional activation and repression. Anti-SPT16 antibody (H-4) enables researchers to gain insights into the dynamics of transcription regulation and the intricate mechanisms that govern gene expression in various biological contexts.
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SPT16 Antibody (H-4) References:
- The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins. | Orphanides, G., et al. 1999. Nature. 400: 284-8. PMID: 10421373
- Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68. | Kang, SW., et al. 2000. Genes Cells. 5: 251-63. PMID: 10792464
- Multiple Nhp6 molecules are required to recruit Spt16-Pob3 to form yFACT complexes and to reorganize nucleosomes. | Ruone, S., et al. 2003. J Biol Chem. 278: 45288-95. PMID: 12952948
- The FACT Spt16 'peptidase' domain is a histone H3-H4 binding module. | Stuwe, T., et al. 2008. Proc Natl Acad Sci U S A. 105: 8884-9. PMID: 18579787
- Histone chaperone spt16 promotes redeposition of the original h3-h4 histones evicted by elongating RNA polymerase. | Jamai, A., et al. 2009. Mol Cell. 35: 377-83. PMID: 19683500
- Structure of the human histone chaperone FACT Spt16 N-terminal domain. | Marcianò, G. and Huang, DT. 2016. Acta Crystallogr F Struct Biol Commun. 72: 121-8. PMID: 26841762
- Proteasomal Regulation of Mammalian SPT16 in Controlling Transcription. | Kaja, A., et al. 2021. Mol Cell Biol. 41: PMID: 33526453
- SPT16 ubiquitylation by DCAF14-CRL4 regulates FACT binding to histones. | Nakagawa, T., et al. 2022. Cell Rep. 38: 110541. PMID: 35320725
- Structural basis for H2A-H2B recognitions by human Spt16. | Li, Y. and Huang, H. 2023. Biochem Biophys Res Commun. 651: 85-91. PMID: 36801613
- The N-terminus of Spt16 anchors FACT to MCM2-7 for parental histone recycling. | Wang, X., et al. 2023. Nucleic Acids Res. 51: 11549-11567. PMID: 37850662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPT16 Antibody (H-4) | sc-165989 | 200 µg/ml | $316.00 | |||
SPT16 Antibody (H-4): m-IgG2b BP-HRP Bundle | sc-548508 | 200 µg Ab; 10 µg BP | $354.00 |