SPT16 Antibody (D-10): sc-165988
- SPT16 Antibody (D-10) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 748-1047 mapping at the C-terminus of SPT16 of human origin
- recommended for detection of SPT16 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for SPT16 Antibody (D-10) for WB applications. These reagents are now offered in bundles with SPT16 Antibody (D-10) (see ordering information below).
SPT16 Antibody (D-10) is a mouse monoclonal IgG2a antibody that detects SPT16 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPT16 is a crucial component of the FACT complex, which plays a significant role in the transcription of chromatin templates by facilitating nucleosome disassembly during transcription elongation. This process is vital for gene expression regulation, as RNA polymerase accesses DNA wrapped around nucleosomes. The FACT complex, consisting of SPT16 and the structure-specific recognition protein-1 (SSRP-1), interacts specifically with histone H2A/H2B dimers, promoting necessary structural changes in chromatin that enable efficient transcription. SPT16′s ability to modulate chromatin structure is essential for proper cellular function and response to various stimuli, making SPT16 a key player in gene expression regulation. Anti-SPT16 antibody (D-10) serves as an invaluable tool for researchers studying transcriptional regulation and chromatin dynamics in various biological contexts.
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SPT16 Antibody (D-10) References:
- Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. | Formosa, T., et al. 2001. EMBO J. 20: 3506-17. PMID: 11432837
- FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. | Heo, K., et al. 2008. Mol Cell. 30: 86-97. PMID: 18406329
- Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. | Kemble, DJ., et al. 2013. J Biol Chem. 288: 10188-94. PMID: 23417676
- The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization. | True, JD., et al. 2016. J Biol Chem. 291: 15307-19. PMID: 27226635
- The structural basis of human Spt16 N-terminal domain interaction with histone (H3-H4)2 tetramer. | Jiang, H., et al. 2019. Biochem Biophys Res Commun. 508: 864-870. PMID: 30528735
- FACT subunit Spt16 controls UVSSA recruitment to lesion-stalled RNA Pol II and stimulates TC-NER. | Wienholz, F., et al. 2019. Nucleic Acids Res. 47: 4011-4025. PMID: 30715484
- Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements. | Ferri, F., et al. 2019. Epigenetics Chromatin. 12: 46. PMID: 31331374
- Evidence that dissociation of Spt16 from transcribed genes is partially dependent on RNA Polymerase II termination. | Campbell, JB., et al. 2019. Transcription. 10: 195-206. PMID: 31809228
- Structure of a histone hexamer bound by the chaperone domains of SPT16 and MCM2. | Gan, S., et al. 2024. Sci China Life Sci. 67: 1305-1307. PMID: 38478295
- Evolution of the chromatin remodeling complex FACT: Functional analysis of SSRP1 and SPT16 in early anther development. | Zhang, Z., et al. 2025. Int J Biol Macromol. 284: 138167. PMID: 39615716
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPT16 Antibody (D-10) | sc-165988 | 200 µg/ml | $322.00 | |||
SPT16 Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-539980 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPT16 Antibody (D-10): m-IgG2a BP-HRP Bundle | sc-546828 | 200 µg Ab; 10 µg BP | $361.00 |