Sprouty 2 Antibody (SQ-5): sc-100862

Sprouty 2 Antibody (SQ-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sprouty 2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Sprouty 2 antibody (SQ-5) is available as a non-conjugated format. Sprouty 2 plays a crucial role as a negative regulator of growth factor signaling, particularly in receptor tyrosine kinases (RTKs). Sprouty 2 inhibits pathways activated by fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF), essential for controlling cell proliferation and migration in normal development and disease states, such as cancer. Sprouty 2 primarily localizes to the cytoplasm and associates with caveolin-1 in perinuclear and vesicular structures, highlighting importance in cellular signaling dynamics. Sprouty 2 undergoes post-translational modifications, including palmitoylation, which facilitates membrane anchoring and influences interactions with other signaling molecules. A well-conserved C-terminal domain of Sprouty 2 contains specific regions necessary for co-localization with microtubules and translocation to membrane ruffles, impacting regulatory roles in embryonic lung morphogenesis and angiogenesis. Sprouty 2 (SQ-5) antibody serves as a valuable tool for studying intricate mechanisms of growth factor signaling and implications in various biological processes.

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Sprouty 2 Antibody (SQ-5) References:

1. Sprouty proteins are targeted to membrane ruffles upon growth factor receptor tyrosine kinase activation. Identification of a novel translocation domain.  |  Lim, J., et al. 2000. J Biol Chem. 275: 32837-45. PMID: 10887178
2. Inhibition of angiogenesis by a mouse sprouty protein.  |  Lee, SH., et al. 2001. J Biol Chem. 276: 4128-33. PMID: 11053436
3. Mammalian sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells.  |  Impagnatiello, MA., et al. 2001. J Cell Biol. 152: 1087-98. PMID: 11238463
4. The C terminus of sprouty is important for modulation of cellular migration and proliferation.  |  Yigzaw, Y., et al. 2001. J Biol Chem. 276: 22742-7. PMID: 11279012
5. Evidence that SPROUTY2 functions as an inhibitor of mouse embryonic lung growth and morphogenesis.  |  Mailleux, AA., et al. 2001. Mech Dev. 102: 81-94. PMID: 11287183
6. ERK pathway positively regulates the expression of Sprouty genes.  |  Ozaki, K., et al. 2001. Biochem Biophys Res Commun. 285: 1084-8. PMID: 11478764
7. Expression and regulation of Sprouty-2 in the granulosa-lutein cells of the corpus luteum.  |  Haimov-Kochman, R., et al. 2005. Mol Hum Reprod. 11: 537-42. PMID: 16126775
8. Immunolocalization of sprouty-1 and sprouty-2 in developing rat lung.  |  Hashimoto, S., et al. 2012. Pathobiology. 79: 34-44. PMID: 22236546
9. Spatiotemporal alterations in Sprouty-2 expression and tyrosine phosphorylation in nitrofen-induced pulmonary hypoplasia.  |  Friedmacher, F., et al. 2013. J Pediatr Surg. 48: 2219-25. PMID: 24210189
10. Sprouty-2 regulates HIV-specific T cell polyfunctionality.  |  Chiu, YL., et al. 2014. J Clin Invest. 124: 198-208. PMID: 24292711
11. Identification of key features required for efficient S-acylation and plasma membrane targeting of sprouty-2.  |  Locatelli, C., et al. 2020. J Cell Sci. 133: PMID: 33037124
12. Sprouty2 limits intestinal tuft and goblet cell numbers through GSK3β-mediated restriction of epithelial IL-33.  |  Schumacher, MA., et al. 2021. Nat Commun. 12: 836. PMID: 33547321

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sprouty 2 Antibody (SQ-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Sprouty 2 Antibody (SQ-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sprouty 2 Antibody (SQ-5) Specifications

Alternate Product Name: Sprouty 2

Product Clone Name: SQ-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-316

Epitope Target: Sprouty 2

Epitope Species: human

Product Reactivity/Detection: Sprouty 2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Sprouty 2 Antibody (SQ-5): N/A

The preferred Sprouty 2 antibody is Sprouty 2 Antibody (D-6): sc-518014.