Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SPRED2 CRISPR/Cas9 KO Plasmid (h): sc-404738
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPRED2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPRED2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPRED2 Antibody (6G8): sc-517018
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPRED2 CRISPR/Cas9 KO Plasmid (h) | sc-404738 | 20 µg | $397.00 |
Overview
SPRED2 (sprouty related EVH1 domain containing 2) encodes a cytosolic adaptor protein that negatively regulates receptor tyrosine kinase signaling by inhibiting the Ras–Raf–MEK–ERK/MAPK cascade. Through interactions with components such as Raf and neurofibromin, SPRED2 helps constrain growth factor–driven proliferation, differentiation, and inflammatory signaling, contributing to homeostatic control of cellular responses. Altered SPRED2 expression or function has been linked to dysregulated MAPK activity in cancer biology and to immune and inflammatory processes, including contexts involving aberrant cytokine and chemokine signaling. As a pathway brake, SPRED2 is frequently studied for its role in tuning signaling amplitude and duration downstream of RTKs and other upstream inputs that converge on ERK.
SPRED2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPRED2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPRED2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPRED2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPRED2 protein expression.
This CRISPR knockout system enables efficient generation of SPRED2-deficient cell models for investigation of SPRED2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPRED2 exon(s) critical for SPRED2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPRED2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPRED2 CRISPR/Cas9 KO Plasmid (h) and SPRED2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPRED2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPRED2 HDR Plasmid (h) and SPRED2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPRED2 homology arms to support homology-directed repair at defined SPRED2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.