SPPL2b CRISPR/Cas9 KO Plasmid (h): sc-405646
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPPL2b CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPPL2b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPPL2b CRISPR/Cas9 KO Plasmid (h) | sc-405646 | 20 µg | $397.00 |
Overview
SPPL2B encodes SPPL2b, an intramembrane aspartyl protease of the signal peptide peptidase–like family that localizes to endosomal and lysosomal membranes. SPPL2b contributes to regulated intramembrane proteolysis, shaping membrane protein turnover and peptide fragment generation within the endo-lysosomal system. Through these activities, SPPL2b is positioned to influence vesicular trafficking, receptor processing, and downstream signaling linked to immune and inflammatory pathways. Dysregulated intramembrane proteolysis and endolysosomal function are relevant to studies of neurodegeneration, immune cell homeostasis, and membrane protein quality control.
SPPL2b CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPPL2B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPPL2B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPPL2B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPPL2b protein expression.
This CRISPR knockout system enables efficient generation of SPPL2B-deficient cell models for investigation of SPPL2b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPPL2B exon(s) critical for SPPL2b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPPL2B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPPL2b CRISPR/Cas9 KO Plasmid (h) and SPPL2b CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPPL2B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPPL2b HDR Plasmid (h) and SPPL2b HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPPL2B homology arms to support homology-directed repair at defined SPPL2B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.