SPLUNC2 CRISPR/Cas9 KO Plasmid (h): sc-406336
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPLUNC2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPLUNC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPLUNC2 CRISPR/Cas9 KO Plasmid (h) | sc-406336 | 20 µg | $397.00 |
Overview
BPIFA2 encodes SPLUNC2, a secreted member of the BPI fold-containing family expressed in upper airway and salivary epithelia. SPLUNC2 contributes to mucosal innate defense by interacting with microbial components and influencing airway surface liquid homeostasis, linking epithelial barrier function to inflammatory signaling programs. Through roles in host–microbe interactions and modulation of local immune responses, BPIFA2 is frequently examined in studies of respiratory tract inflammation, infection susceptibility, and epithelial remodeling. Altered BPIFA2/SPLUNC2 expression has been associated with airway disease phenotypes and is used as a molecular readout in models of sinonasal and pulmonary inflammatory conditions.
SPLUNC2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BPIFA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BPIFA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BPIFA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPLUNC2 protein expression.
This CRISPR knockout system enables efficient generation of BPIFA2-deficient cell models for investigation of SPLUNC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BPIFA2 exon(s) critical for SPLUNC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BPIFA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPLUNC2 CRISPR/Cas9 KO Plasmid (h) and SPLUNC2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BPIFA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPLUNC2 HDR Plasmid (h) and SPLUNC2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BPIFA2 homology arms to support homology-directed repair at defined BPIFA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.