Spastin Antibody (Sp 3G11/1): sc-53443

Spastin Antibody (Sp 3G11/1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Spastin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Spastin antibody (Sp 3G11/1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Spastin, a member of the AAA protein family, consists of 616 amino acids and plays a crucial role in microtubule dynamics, which are essential for maintaining cellular structure and facilitating intracellular transport. Proper functioning of Spastin is vital for nuclear protein complex assembly and function, and dysregulation can lead to significant cellular dysfunction. Notably, mutations in the Spastin gene (SPAST, SPG4) are linked to the most prevalent form of hereditary spastic paraplegia, specifically spastic paraplegia 4, which is characterized by progressive weakness and stiffness in the lower limbs due to retrograde axonopathy affecting corticospinal motor neurons. Approximately 40% of all autosomal dominant hereditary spastic paraplegias are attributed to SPAST-specific mutations, underscoring Spastin′s importance in neurological health.

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Spastin Antibody (Sp 3G11/1) References:

1. Hereditary spastic paraplegia with cerebellar ataxia: a complex phenotype associated with a new SPG4 gene mutation.  |  Nielsen, JE., et al. 2004. Eur J Neurol. 11: 817-24. PMID: 15667412
2. Subcellular localization of spastin: implications for the pathogenesis of hereditary spastic paraplegia.  |  Svenson, IK., et al. 2005. Neurogenetics. 6: 135-41. PMID: 15891913
3. Reduced regional cerebral blood flow in SPG4-linked hereditary spastic paraplegia.  |  Scheuer, KH., et al. 2005. J Neurol Sci. 235: 23-32. PMID: 15939438
4. Spastin related hereditary spastic paraplegia with dysplastic corpus callosum.  |  Alber, B., et al. 2005. J Neurol Sci. 236: 9-12. PMID: 16009377
5. Early-onset ALS with long-term survival associated with spastin gene mutation.  |  Meyer, T., et al. 2005. Neurology. 65: 141-3. PMID: 16009903
6. Spastin subcellular localization is regulated through usage of different translation start sites and active export from the nucleus.  |  Claudiani, P., et al. 2005. Exp Cell Res. 309: 358-69. PMID: 16026783
7. Spastin mutations are frequent in sporadic spastic paraparesis and their spectrum is different from that observed in familial cases.  |  Depienne, C., et al. 2006. J Med Genet. 43: 259-65. PMID: 16055926
8. Thin corpus callosum and amyotrophy in spastic paraplegia--case report and review of literature.  |  Winner, B., et al. 2006. Clin Neurol Neurosurg. 108: 692-8. PMID: 16102895
9. Tau missorting and spastin-induced microtubule disruption in neurodegeneration: Alzheimer Disease and Hereditary Spastic Paraplegia.  |  Zempel, H. and Mandelkow, EM. 2015. Mol Neurodegener. 10: 68. PMID: 26691836
10. Spastin Interacts with CRMP2 to Regulate Neurite Outgrowth by Controlling Microtubule Dynamics through Phosphorylation Modifications.  |  Li, S., et al. 2021. CNS Neurol Disord Drug Targets. 20: 249-265. PMID: 33109053
11. The Role of Spastin in Axon Biology.  |  Costa, AC. and Sousa, MM. 2022. Front Cell Dev Biol. 10: 934522. PMID: 35865632
12. Cul-4 inhibition rescues spastin levels and reduces defects in hereditary spastic paraplegia models.  |  Sardina, F., et al. 2024. Brain. 147: 3534-3546. PMID: 38551087

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Spastin Antibody (Sp 3G11/1) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-Spastin Antibody (Sp 3G11/1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Spastin Antibody (Sp 3G11/1) Specifications

Alternate Product Name: Spastin

Product Clone Name: Sp 3G11/1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Spastin

Epitope Species: human

Product Reactivity/Detection: Spastin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for Spastin Antibody (Sp 3G11/1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin