Spastin Antibody (C-3): sc-374068

Spastin Antibody (C-3) is a mouse monoclonal IgG3 kappa light chain antibody that detects Spastin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Spastin antibody (C-3) is available as a non-conjugated format. Spastin, a member of the AAA protein family, is a 616 amino acid protein that plays a crucial role in microtubule dynamics, which is essential for maintaining cellular structure and function. Spastin (C-3) antibody recognizes nuclear and cytoplasmic locations, where Spastin is involved in microtubule disassembly, a process vital for various cellular activities, including cell division and intracellular transport. Disruption of microtubule dynamics can lead to severe cellular dysfunction, highlighting Spastin′s importance in maintaining cellular integrity. Mutations in the Spastin gene (SPAST, SPG4) are linked to the most common form of spastic paraplegia 4, an autosomal dominant hereditary spastic paraplegia characterized by progressive weakness and stiffness in the lower limbs due to retrograde axonopathy of corticospinal motor neurons. Approximately 40% of all autosomal dominant hereditary spastic paraplegias are attributed to SPAST-specific mutations, underscoring Spastin′s significance in neurological health.

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Spastin Antibody (C-3) References:

1. The microtubule-severing proteins spastin and katanin participate differently in the formation of axonal branches.  |  Yu, W., et al. 2008. Mol Biol Cell. 19: 1485-98. PMID: 18234839
2. The nucleotide cycle of spastin correlates with its microtubule-binding properties.  |  Wen, M. and Wang, C. 2013. FEBS J. 280: 3868-77. PMID: 23745751
3. Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and axon development.  |  Goyal, U., et al. 2014. PLoS One. 9: e112428. PMID: 25390646
4. Effect of exogenous spastin combined with polyethylene glycol on sciatic nerve injury.  |  Lin, YF., et al. 2019. Neural Regen Res. 14: 1271-1279. PMID: 30804259
5. HIPK2 Phosphorylates the Microtubule-Severing Enzyme Spastin at S268 for Abscission.  |  Pisciottani, A., et al. 2019. Cells. 8: PMID: 31284535
6. ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.  |  Connell, JW., et al. 2020. Cell Mol Life Sci. 77: 2641-2658. PMID: 31587092
7. Spastin mutations impair coordination between lipid droplet dispersion and reticulum.  |  Arribat, Y., et al. 2020. PLoS Genet. 16: e1008665. PMID: 32315314
8. Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis.  |  Schweigel, U., et al. 2022. Nucleus. 13: 144-154. PMID: 35298348
9. Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein.  |  Shen, W., et al. 2023. Virol Sin. 38: 448-458. PMID: 37172824
10. Targeting MDM2 affects spastin protein levels and functions: implications for HSP treatment.  |  Sardina, F., et al. 2025. Cell Death Discov. 11: 53. PMID: 39920118

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Spastin Antibody (C-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Spastin Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Spastin Antibody (C-3) Specifications

Alternate Product Name: Spastin

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 289-317

Epitope Target: Spastin

Epitope Species: human

Product Reactivity/Detection: Spastin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Spastin Antibody (C-3): P: Blocking Peptide

The preferred Spastin antibody is Spastin Antibody (Sp 3G11/1): sc-53443.