SPAK Antibody (430CT4.4.1): sc-517361

SPAK Antibody (430CT4.4.1) is a mouse monoclonal IgG1 antibody that detects SPAK protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-SPAK antibody (430CT4.4.1) is available as a non-conjugated format. SPAK, also known as STK39 (serine threonine kinase 39), DCHT, or PASK, is a critical serine/threonine protein kinase composed of 547 amino acids, which plays a vital role in cellular signaling pathways. SPAK is predominantly expressed in the pancreas, brain, heart, lung, liver, and testis, and localizes in both the cytoplasm and nucleus, where SPAK contains a single protein kinase domain. SPAK catalyzes ATP-dependent phosphorylation of target proteins, mediating stress-activated signals essential for regulating various cellular functions, including cell division, homeostasis, and apoptosis. The phosphorylation and dephosphorylation of proteins on serine and threonine residues are crucial for maintaining cellular integrity and responding to environmental changes. The gene encoding SPAK is located on human chromosome 2, which contains over 1,400 genes and represents nearly 8% of the human genome, highlighting SPAK′s significance in human health and disease.

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SPAK Antibody (430CT4.4.1) References:

1. SPAK, a STE20/SPS1-related kinase that activates the p38 pathway.  |  Johnston, AM., et al. 2000. Oncogene. 19: 4290-7. PMID: 10980603
2. Androgens induce expression of SPAK, a STE20/SPS1-related kinase, in LNCaP human prostate cancer cells.  |  Qi, H., et al. 2001. Mol Cell Endocrinol. 182: 181-92. PMID: 11514053
3. PASK (proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1).  |  Dowd, BF. and Forbush, B. 2003. J Biol Chem. 278: 27347-53. PMID: 12740379
4. Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase.  |  Piechotta, K., et al. 2003. J Biol Chem. 278: 52848-56. PMID: 14563843
5. WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1.  |  Moriguchi, T., et al. 2005. J Biol Chem. 280: 42685-93. PMID: 16263722
6. Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1.  |  Vitari, AC., et al. 2006. Biochem J. 397: 223-31. PMID: 16669787
7. TRAIL-induced cleavage and inactivation of SPAK sensitizes cells to apoptosis.  |  Polek, TC., et al. 2006. Biochem Biophys Res Commun. 349: 1016-24. PMID: 16950202
8. Nuclear factor-kappaB is a critical mediator of Ste20-like proline-/alanine-rich kinase regulation in intestinal inflammation.  |  Yan, Y., et al. 2008. Am J Pathol. 173: 1013-28. PMID: 18787102