SPAG17 CRISPR/Cas9 KO Plasmid (h): sc-410482
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPAG17 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPAG17 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPAG17 CRISPR/Cas9 KO Plasmid (h) | sc-410482 | 20 µg | $397.00 |
Overview
SPAG17 encodes sperm associated antigen 17, a structural and regulatory component of the axonemal apparatus that supports motile cilia and flagella function. It participates in microtubule-based processes and contributes to dynein-driven beating mechanics required for effective cellular motility and fluid propulsion across epithelia. In human biology, altered SPAG17 expression or disruption is linked to defects in spermatogenesis and sperm motility and is relevant to broader ciliopathy-associated phenotypes involving impaired ciliary structure and signaling. As a cilia/flagella-associated protein, SPAG17 is also useful for probing how cytoskeletal organization interfaces with cell-cycle dynamics and tissue-specific differentiation programs.
SPAG17 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPAG17 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPAG17 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPAG17 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPAG17 protein expression.
This CRISPR knockout system enables efficient generation of SPAG17-deficient cell models for investigation of SPAG17 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPAG17 exon(s) critical for SPAG17 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPAG17 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPAG17 CRISPR/Cas9 KO Plasmid (h) and SPAG17 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPAG17 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPAG17 HDR Plasmid (h) and SPAG17 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPAG17 homology arms to support homology-directed repair at defined SPAG17 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.