SPACA1 CRISPR/Cas9 KO Plasmid (h): sc-406974
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPACA1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SPACA1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPACA1 CRISPR/Cas9 KO Plasmid (h) | sc-406974 | 20 µg | $397.00 |
Overview
SPACA1 (sperm acrosome associated 1) encodes an acrosomal protein implicated in sperm head morphogenesis and the biogenesis/organization of acrosomal membranes during spermiogenesis. Its function is linked to male germ cell differentiation processes that depend on coordinated vesicle trafficking, membrane remodeling, and cytoskeletal organization to support acrosome formation and fertilization competency. Altered SPACA1 activity has been associated with defects in sperm structure and reduced male fertility in experimental settings, making it relevant for studying mechanisms of spermatogenic failure. As a testis-enriched factor, SPACA1 is also useful as a marker and functional node in pathways governing gamete development and reproductive biology.
SPACA1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPACA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPACA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPACA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SPACA1 protein expression.
This CRISPR knockout system enables efficient generation of SPACA1-deficient cell models for investigation of SPACA1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPACA1 exon(s) critical for SPACA1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPACA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SPACA1 CRISPR/Cas9 KO Plasmid (h) and SPACA1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPACA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SPACA1 HDR Plasmid (h) and SPACA1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPACA1 homology arms to support homology-directed repair at defined SPACA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.