SP140 CRISPR/Cas9 KO Plasmid (h): sc-409816
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SP140 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SP140 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SP140 CRISPR/Cas9 KO Plasmid (h) | sc-409816 | 20 µg | $397.00 |
Overview
SP140 encodes a nuclear chromatin-associated protein predominantly expressed in hematopoietic and immune lineages, where it contributes to transcriptional regulation and cell state control. As a member of the SP100/SP140 family containing SAND, PHD, and bromodomain-like modules, SP140 is linked to recognition of chromatin features and assembly of regulatory complexes that tune inflammatory and differentiation programs. It has been implicated in innate and adaptive immune signaling pathways, including modulation of cytokine-driven transcriptional responses and macrophage activation states. Genetic and functional studies associate altered SP140 activity with immune-mediated disorders, making it a useful target for dissecting mechanisms of immune gene regulation and epigenetic control.
SP140 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SP140 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SP140 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SP140 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SP140 protein expression.
This CRISPR knockout system enables efficient generation of SP140-deficient cell models for investigation of SP140 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SP140 exon(s) critical for SP140 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SP140 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SP140 CRISPR/Cas9 KO Plasmid (h) and SP140 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SP140 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SP140 HDR Plasmid (h) and SP140 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SP140 homology arms to support homology-directed repair at defined SP140 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.