Sp110 Double Nickase Plasmid (h): sc-405542-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sp110 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sp110 Double Nickase Plasmid (h) and Sp110 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SP110. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sp110 Antibody (B-10): sc-376741
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sp110 Double Nickase Plasmid (h) | sc-405542-NIC | 20 µg | $410.00 | |||
Sp110 Double Nickase Plasmid (h2) | sc-405542-NIC-2 | 20 µg | $410.00 |
SP110 encodes Sp110, a nuclear body–associated protein implicated in chromatin-dependent transcriptional control and innate immune regulation. Sp110 is linked to interferon-responsive gene programs and macrophage activation states, and it is thought to modulate nuclear architecture and transcription factor availability within promyelocytic leukemia (PML) nuclear bodies. Genetic and functional studies associate SP110 with susceptibility to mycobacterial infection and inflammatory immune phenotypes, highlighting its relevance to host–pathogen interactions. As a regulator of immune transcriptional networks, SP110 is frequently examined in pathways governing cytokine signaling, antigen presentation, and cell-intrinsic antimicrobial responses.
Sp110 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SP110 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SP110. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SP110 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SP110-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.