Sox10 Double Nickase Plasmid (m): sc-423078-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox10 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sox10 Double Nickase Plasmid (m) and Sox10 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sox10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox10 Antibody (A-2): sc-365692
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox10 Double Nickase Plasmid (m) | sc-423078-NIC | 20 µg | $410.00 | |||
Sox10 Double Nickase Plasmid (m2) | sc-423078-NIC-2 | 20 µg | $410.00 |
Mouse Sox10 encodes an HMG-box transcription factor that controls neural crest lineage specification and the differentiation and maintenance of myelinating glia, including Schwann cells and oligodendrocytes. Sox10 coordinates transcriptional programs with partners such as PAX3 and SOX9 to regulate genes involved in myelin formation, peripheral nerve development, and melanocyte biology. Through these networks, Sox10 influences processes spanning cell fate commitment, migration, and maturation, and its dysregulation is linked to congenital neurocristopathies and pigmentation or enteric nervous system defects. In cancer biology, altered SOX10-dependent gene expression is widely used to study melanoma lineage state, invasion programs, and transcriptional plasticity in relevant model systems.
Sox10 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sox10 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sox10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sox10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sox10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.