SOBP CRISPR/Cas9 KO Plasmid (h): sc-412439
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SOBP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SOBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SOBP CRISPR/Cas9 KO Plasmid (h) | sc-412439 | 20 µg | $397.00 |
Overview
SOBP (sine oculis-binding protein) is a nuclear protein implicated in transcriptional regulation programs required for inner ear and sensory system development, with roles in cell fate specification and tissue patterning. Human genetic evidence links loss-of-function variants in SOBP to autosomal recessive deafness and neurodevelopmental phenotypes, highlighting its importance in auditory pathway formation and neuronal maturation. Perturbation of SOBP is therefore relevant for studying gene regulatory networks that coordinate differentiation, morphogenesis, and sensory organ function. In cellular models, SOBP disruption can be used to interrogate downstream transcriptional responses and developmental signaling processes that shape sensory lineage commitment.
SOBP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOBP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOBP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOBP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SOBP protein expression.
This CRISPR knockout system enables efficient generation of SOBP-deficient cell models for investigation of SOBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOBP exon(s) critical for SOBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOBP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SOBP CRISPR/Cas9 KO Plasmid (h) and SOBP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOBP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SOBP HDR Plasmid (h) and SOBP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOBP homology arms to support homology-directed repair at defined SOBP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.