SOAT1 CRISPR/Cas9 KO Plasmid (m): sc-423068
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SOAT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SOAT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SOAT1 Antibody (A-11): sc-136959
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SOAT1 CRISPR/Cas9 KO Plasmid (m) | sc-423068 | 20 µg | $397.00 |
Overview
Mouse Soat1 encodes sterol O-acyltransferase 1 (SOAT1), an endoplasmic reticulum membrane enzyme that esterifies free cholesterol to cholesteryl esters using long-chain fatty acyl-CoA substrates. This reaction supports cholesterol homeostasis, lipid droplet biogenesis, and membrane lipid remodeling, linking SOAT1 to cellular lipid storage and ER stress responses. SOAT1 activity intersects with cholesterol trafficking and lipoprotein metabolism pathways and can influence foam cell formation and macrophage lipid handling. Dysregulated SOAT1-dependent cholesteryl ester accumulation has been associated with metabolic inflammation and lipid-driven pathology, making Soat1 a useful target for mechanistic studies in atherosclerosis-related and metabolic disease models.
SOAT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Soat1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Soat1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Soat1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SOAT1 protein expression.
This CRISPR knockout system enables efficient generation of Soat1-deficient cell models for investigation of SOAT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Soat1 exon(s) critical for SOAT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Soat1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SOAT1 CRISPR/Cas9 KO Plasmid (m) and SOAT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Soat1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SOAT1 HDR Plasmid (m) and SOAT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Soat1 homology arms to support homology-directed repair at defined Soat1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.