SOAT1 Antibody (ACAT-1): sc-69836

SOAT1 Antibody (ACAT-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SOAT1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-SOAT1 antibody (ACAT-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SOAT1, also known as sterol O-acyltransferase-1, is a homotetrameric enzyme that plays a crucial role in lipid metabolism by catalyzing the conversion of cholesterol and long-chain fatty acyl-coenzyme A into cholesterol esters. This enzymatic activity is vital for maintaining cellular cholesterol homeostasis, which is essential for various cellular functions, including membrane integrity and signaling. The gene encoding human SOAT1 is located on chromosome 1 and is predominantly expressed in the endoplasmic reticulum of tissues such as the liver, kidney, adrenal glands, and macrophages. Dysregulation of SOAT1 activity is implicated in the formation of foam cells, a key event in the development of atherosclerosis, making SOAT1 a target for therapeutic intervention. Additionally, SOAT1 interacts with other proteins involved in lipid metabolism, further underscoring SOAT1′s importance in cellular lipid regulation and potential implications in cardiovascular diseases.

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SOAT1 Antibody (ACAT-1) References:

1. Human acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) gene organization and evidence that the 4.3-kilobase ACAT-1 mRNA is produced from two different chromosomes.  |  Li, BL., et al. 1999. J Biol Chem. 274: 11060-71. PMID: 10196189
2. Human acyl-CoA:cholesterol acyltransferase-1 in the endoplasmic reticulum contains seven transmembrane domains.  |  Lin, S., et al. 1999. J Biol Chem. 274: 23276-85. PMID: 10438503
3. Human acyl-CoA:cholesterol acyltransferase-1 is a homotetrameric enzyme in intact cells and in vitro.  |  Yu, C., et al. 1999. J Biol Chem. 274: 36139-45. PMID: 10593897
4. Immunological quantitation and localization of ACAT-1 and ACAT-2 in human liver and small intestine.  |  Chang, CC., et al. 2000. J Biol Chem. 275: 28083-92. PMID: 10846185
5. Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D(3) or 9-cis-retinoic acid in undifferentiated THP-1 cells.  |  Maung, K., et al. 2001. J Lipid Res. 42: 181-7. PMID: 11181746
6. Synergistic transcriptional activation of human Acyl-coenzyme A: cholesterol acyltransterase-1 gene by interferon-gamma and all-trans-retinoic acid THP-1 cells.  |  Yang, JB., et al. 2001. J Biol Chem. 276: 20989-98. PMID: 11399774
7. Direct effect of an acyl-CoA:cholesterol acyltransferase inhibitor, F-1394, on atherosclerosis in apolipoprotein E and low density lipoprotein receptor double knockout mice.  |  Chiwata, T., et al. 2001. Br J Pharmacol. 133: 1005-12. PMID: 11487509
8. Acyl-coenzyme A: cholesterol acyltransferase modulates the generation of the amyloid beta-peptide.  |  Puglielli, L., et al. 2001. Nat Cell Biol. 3: 905-12. PMID: 11584272
9. High-affinity SOAT1 ligands remodeled cholesterol metabolism program to inhibit tumor growth.  |  Wang, Z., et al. 2022. BMC Med. 20: 292. PMID: 35941608
10. SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma.  |  Fu, R., et al. 2024. Cell Death Dis. 15: 325. PMID: 38724499
11. ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization.  |  Liu, Y., et al. 2024. Int J Biol Sci. 20: 3372-3392. PMID: 38993570
12. Recombinant acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner.  |  Chang, CC., et al. 1998. J Biol Chem. 273: 35132-41. PMID: 9857049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SOAT1 Antibody (ACAT-1) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-SOAT1 Antibody (ACAT-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SOAT1 Antibody (ACAT-1) Specifications

Alternate Product Name: SOAT1

Product Clone Name: ACAT-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-131

Epitope Target: SOAT1

Epitope Species: human

Product Reactivity/Detection: SOAT1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for SOAT1 Antibody (ACAT-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin