Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNX9 Double Nickase Plasmid (h): sc-403578-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNX9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SNX9 Double Nickase Plasmid (h) and SNX9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SNX9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNX9 Antibody (G-5): sc-166863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX9 Double Nickase Plasmid (h) | sc-403578-NIC | 20 µg | $410.00 | |||
SNX9 Double Nickase Plasmid (h2) | sc-403578-NIC-2 | 20 µg | $410.00 |
SNX9 (sorting nexin 9) is a PX-BAR domain adaptor that couples phosphoinositide recognition to membrane remodeling and endocytic trafficking. It participates in clathrin-mediated endocytosis and vesicle budding by coordinating curvature sensing with recruitment of actin regulators and dynamin during vesicle scission. Through these functions, SNX9 influences receptor internalization, signaling attenuation, and cytoskeletal dynamics, processes relevant to cell migration and proliferative signaling networks. Altered SNX9-dependent trafficking has been studied in the context of oncogenic signaling, neurobiology, and pathogen entry pathways where endocytic control impacts cellular homeostasis.
SNX9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SNX9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SNX9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SNX9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SNX9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.