SNX9 Antibody (G-5) recommended for detection of SNX9 of mouse, rat and human origin by WB, IP, IF and ELISA

SNX9 Antibody (G-5): sc-166863

SNX9 Antibody (G-5) is rated 4.3 out of 5 by 6.
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Datasheets

    • Anti-SNX9 Antibody (G-5) is a mouse monoclonal IgG1 (kappa light chain) SNX9 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 391-436 within an internal region of SNX9 of human origin
    • Anti-SNX9 Antibody (G-5) is recommended for detection of SNX9 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SNX9 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SNX9 (G-5): sc-166863.

What is the recommended starting dilution for Western blot for sc-166863: SNX9 (G-5) monoclonal antibody?

Asked by: jenni
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from No bands in WB I did a 1:200 dilution of antibody with milk 5% in TBS-T. I left it overnight incubation, and when revealing, no bands appeared in the membrane.
Date published: 2018-02-15
Rated 5 out of 5 by from Good features We have test it by IF in HeLa cells, we got a dots like structure in cell and no staining for the nuclei of cells which is consistent with our transfected data.
Date published: 2017-09-25
Rated 5 out of 5 by from Very goood I used sample vial I requested from SCBT. I tried endogenous SNX9 expression of 293T cell with different plasmids of interests.
Date published: 2017-07-11
Rated 5 out of 5 by from Good detection of mouse and human SNX9 This antibody works great in Western Blot, showing a single band of good strength at a consistent weight with both mouse and human cell lines.
Date published: 2017-02-03
Rated 5 out of 5 by from Worked well for Western with human SNX9 transfected Worked well for Western with human SNX9 transfected lysate. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Publishable data with monkey BSC1 Publishable data with monkey BSC1 - SCBT Publication Review
Date published: 2013-01-05
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