SNX17 Antibody (H-10) recommended for detection of SNX17 of mouse, rat and human origin by WB, IP, IF and ELISA

SNX17 Antibody (H-10): sc-166957

SNX17 Antibody (H-10) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-SNX17 Antibody (H-10) is a mouse monoclonal IgG1 (kappa light chain) SNX17 antibody provided at 200 µg/ml
    • raised against amino acids 270-470 mapping at the C-terminus of SNX17 of human origin
    • Anti-SNX17 Antibody (H-10) is recommended for detection of SNX17 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SNX17 Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SNX17 (H-10): sc-166957.

What is the recommended starting dilution for Western blot for sc-166957: SNX17 (H-10) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22

En un WB usando BMDC se me revelan dos línas de SNX17, una de 55 K y otra banda menos marcada de unos 60 K. Además, trabajo con dos líneas de SNX17 KD y también observo estas dos líneas, ambas se atenúan con el silenciamiento. ¿A qué puede deberse?Thanks

Asked by: Asturiana
Gracias por su pregunta. Hemos probado nuestros anticuerpos SNX17 en una variedad de diferentes líneas celulares y no hemos observado un patrón de bandas similar en Western blot. Recomendamos probar las mismas muestras usando un anticuerpo SNX17 diferente con un epítopo distinto para ver si observa el mismo resultado. Actualmente ofrecemos otros dos anticuerpos monoclonales de ratón a SNX17, sc-166597: SNX17 (E-12), que tiene un epítopo C-terminal, y sc-166585: SNX17 (D-10), que tiene un epítopo N-terminal. También recomendamos consultar la literatura primaria para ver si otros investigadores han observado un resultado similar con el antígeno SNX17. Thank you for your question. We have tested our SNX17 antibodies in a variety of different cell lines and have not observed a similar banding pattern in Western blot. We would recommend testing the same samples using a different SNX17 antibody with a distinct epitope to see if you observe the same result. We currently offer two other mouse monoclonal antibodies to SNX17, sc-166597: SNX17 (E-12), which has a C-terminal epitope, and sc-166585: SNX17 (D-10), which has an N-terminal epitope. We also recommend consulting the primary literature to see if any other researchers have observed a similar result with the SNX17 antigen.
Answered by: Tech Service 3
Date published: 2016-12-21
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Rated 5 out of 5 by from very effective Our lab analyzed SNX17 antibodies with WB, and we got all of them in 53kDa. In addition, the antibody can be reused.
Date published: 2017-10-26
Rated 5 out of 5 by from Clear band in WB Work well in WB of mouse cardiomyocytes and cardiac tissue!
Date published: 2017-06-28
Rated 5 out of 5 by from Good strong band Nice, strong band observed with SNX17 Antibody (H-10) in various samples
Date published: 2017-02-01
Rated 5 out of 5 by from Beautiful strong band in PC Beautiful strong band in PC-3 whole cell lysate. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Worked well with mouse and human SNX17 transfected Worked well with mouse and human SNX17 transfected lysates by WB -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Great monoclonal antibody Great monoclonal antibody, good specificity and strong signal strength in HeLa, BJAB, A-431, K-562, Jurkat and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-01-13
Rated 5 out of 5 by from WB: Strong band observed using ES WB: Strong band observed using ES-2 whole cell lysate. -SCBT QC
Date published: 2013-09-03
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