Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SNX1 Double Nickase Plasmid (h): sc-404470-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SNX1 Double Nickase Plasmid (h) and SNX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SNX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNX1 Antibody (B-8): sc-376376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX1 Double Nickase Plasmid (h) | sc-404470-NIC | 20 µg | $410.00 | |||
SNX1 Double Nickase Plasmid (h2) | sc-404470-NIC-2 | 20 µg | $410.00 |
Sorting nexin 1 (SNX1) is a PX domain–containing endosomal protein that binds phosphatidylinositol 3-phosphate and cooperates with the retromer complex to regulate endosomal sorting and retrograde transport from endosomes to the trans-Golgi network. By shaping endosomal membrane tubules and influencing receptor trafficking, SNX1 helps control the recycling and downregulation of multiple signaling receptors, linking it to pathways such as EGFR and other receptor tyrosine kinase networks. SNX1-dependent trafficking impacts cellular homeostasis processes including membrane protein turnover, nutrient uptake, and signaling attenuation. Dysregulated SNX1 expression or function has been associated with altered receptor signaling and endosomal dynamics reported across cancer biology and neurodegeneration-related research contexts.
SNX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SNX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SNX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SNX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SNX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.