SNAPC 190 Antibody (B-11): sc-515660
- SNAPC 190 Antibody (B-11) is a mouse monoclonal IgG1 κ SNAPC 190 antibody provided at 200 µg/ml
- raised against amino acids 147-446 mapping within an internal region of SNAPC 190 of human origin
- SNAPC 190 Antibody (B-11) is recommended for detection of SNAPC 190 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SNAPC 190 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SNAPC 190 Antibody (B-11) for WB applications. These reagents are now offered in bundles with SNAPC 190 Antibody (B-11) (see ordering information below).
QUICK LINKS
SNAPC 190 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SNAPC 190 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SNAPC 190 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SNAPC 190 plays a crucial role in gene transcription regulation, particularly in assembling the snRNA-activating protein complex (SNAPC), which is essential for small nuclear RNA transcription by RNA polymerases II and III. SNAPC 190 is composed of five subunits of varying sizes, and SNAPC 190′s interaction with TATA-box binding protein (TBP) and TBP-associated factors (TAFs) is vital for transcriptional machinery. Oct-1′s POU domain binds to SNAPC 190, facilitating SNAPC recruitment to a non-TATA-box proximal sequence element, which is critical for basal transcription regulation. Cooperative binding of SNAPC and Oct-1 is enhanced by a nucleosome positioned between their respective binding sites, underscoring chromatin structure′s importance in transcription regulation. SNAPC 190 also drives SNAPC complex assembly, necessary for effective transcriptional activation, highlighting SNAPC 190′s significance in gene expression and cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SNAPC 190 Antibody (B-11) References:
- A positioned nucleosome on the human U6 promoter allows recruitment of SNAPc by the Oct-1 POU domain. | Zhao, X., et al. 2001. Mol Cell. 7: 539-49. PMID: 11463379
- A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerase II and III. | Henry, RW., et al. 1995. Nature. 374: 653-6. PMID: 7715707
- Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE. | Sadowski, CL., et al. 1993. Genes Dev. 7: 1535-48. PMID: 8339931
- The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein. | Sadowski, CL., et al. 1996. Proc Natl Acad Sci U S A. 93: 4289-93. PMID: 8633057
- Cloning and characterization of SNAP50, a subunit of the snRNA-activating protein complex SNAPc. | Henry, RW., et al. 1996. EMBO J. 15: 7129-36. PMID: 9003788
- Role for the amino-terminal region of human TBP in U6 snRNA transcription. | Mittal, V. and Hernandez, N. 1997. Science. 275: 1136-40. PMID: 9027316
- Characterization of a trimeric complex containing Oct-1, SNAPc, and DNA. | Ford, E. and Hernandez, N. 1997. J Biol Chem. 272: 16048-55. PMID: 9188510
- SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III. | Henry, RW., et al. 1998. Genes Dev. 12: 2664-72. PMID: 9732265
- The Oct-1 POU domain activates snRNA gene transcription by contacting a region in the SNAPc largest subunit that bears sequence similarities to the Oct-1 coactivator OBF-1. | Ford, E., et al. 1998. Genes Dev. 12: 3528-40. PMID: 9832505
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAPC 190 Antibody (B-11) | sc-515660 | 200 µg/ml | $322.00 | |||
SNAPC 190 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-528054 | 200 µg Ab; 10 µg BP | $361.00 | |||
SNAPC 190 Antibody (B-11): m-IgG1 BP-HRP Bundle | sc-533427 | 200 µg Ab; 20 µg BP | $361.00 | |||
SNAPC 190 Antibody (B-11) AC | sc-515660 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SNAPC 190 Antibody (B-11) HRP | sc-515660 HRP | 200 µg/ml | $322.00 | |||
SNAPC 190 Antibody (B-11) FITC | sc-515660 FITC | 200 µg/ml | $336.00 | |||
SNAPC 190 Antibody (B-11) PE | sc-515660 PE | 200 µg/ml | $349.00 | |||
SNAPC 190 Antibody (B-11) Alexa Fluor® 488 | sc-515660 AF488 | 200 µg/ml | $364.00 | |||
SNAPC 190 Antibody (B-11) Alexa Fluor® 546 | sc-515660 AF546 | 200 µg/ml | $364.00 | |||
SNAPC 190 Antibody (B-11) Alexa Fluor® 594 | sc-515660 AF594 | 200 µg/ml | $364.00 | |||
SNAPC 190 Antibody (B-11) Alexa Fluor® 647 | sc-515660 AF647 | 200 µg/ml | $364.00 | |||
SNAPC 190 Antibody (B-11) Alexa Fluor® 680 | sc-515660 AF680 | 200 µg/ml | $364.00 | |||
SNAPC 190 Antibody (B-11) Alexa Fluor® 790 | sc-515660 AF790 | 200 µg/ml | $364.00 |