SNAP47 Antibody (D-11): sc-514428
- SNAP47 Antibody (D-11) is a mouse monoclonal IgG1 κ SNAP47 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 290-442 mapping near the C-terminus of SNAP47 of human origin
- SNAP47 Antibody (D-11) is recommended for detection of SNAP47 of human origin by WB, IP, IF and ELISA
- Anti-SNAP47 Antibody (D-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SNAP47 Antibody (D-11) for WB applications. These reagents are now offered in bundles with SNAP47 Antibody (D-11) (see ordering information below).
SNAP47 Antibody (D-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SNAP47 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SNAP47 antibody (D-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SNAP47, also known as synaptosomal-associated protein 47 or epididymis luminal protein 170, is a 464 amino acid protein that plays a crucial role in vesicular transport within eukaryotic cells. SNAP47 is predominantly expressed in nervous tissue and is essential for SNARE complex formation, which is vital for vesicle fusion with target membranes. This process ensures accurate protein transport to intended cellular locations. SNAP47 interaction with other SNARE proteins facilitates recruitment of soluble NSF attachment proteins and N-ethylmaleimide-sensitive factor, which together stabilize SNARE complex and drive lipid bilayer fusion. SNAP47 exists in four isoforms due to alternative splicing, which may contribute to diverse functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SNAP47 Antibody (D-11) References:
- Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression. | Holt, M., et al. 2006. J Biol Chem. 281: 17076-17083. PMID: 16621800
- Membrane fusion in cells: molecular machinery and mechanisms. | Leabu, M. 2006. J Cell Mol Med. 10: 423-7. PMID: 16796809
- Core proteins of the secretory machinery. | Lang, T. and Jahn, R. 2008. Handb Exp Pharmacol. 107-27. PMID: 18064413
- Assembly and disassembly of SNAREs in membrane fusion. | Jena, BP. 2008. Methods Cell Biol. 90: 157-82. PMID: 19195550
- Engineering botulinum neurotoxin to extend therapeutic intervention. | Chen, S. and Barbieri, JT. 2009. Proc Natl Acad Sci U S A. 106: 9180-4. PMID: 19487672
- Helical extension of the neuronal SNARE complex into the membrane. | Stein, A., et al. 2009. Nature. 460: 525-8. PMID: 19571812
- Membrane fusion: role of SNAREs and calcium. | Jena, BP. 2009. Protein Pept Lett. 16: 712-7. PMID: 19601899
- Transmembrane-domain determinants for SNARE-mediated membrane fusion. | Fdez, E., et al. 2010. J Cell Sci. 123: 2473-80. PMID: 20571052
- Differential effect of exocytic SNAREs on the production of recombinant proteins in mammalian cells. | Peng, RW., et al. 2011. Biotechnol Bioeng. 108: 611-20. PMID: 21246508
- SNAP47 Interacts with ATG14 to Promote VP1 Conjugation and CVB3 Propagation. | Xiang, P., et al. 2021. Cells. 10: PMID: 34440910
- Circ-SNAP47 (hsa_circ_0016760) and miR-625-5p are regulators of WEE1 in regulation of chemoresistance, growth and invasion of DDP-tolerant NSCLC cells via ceRNA pathway. | Zou, C., et al. 2022. Environ Toxicol. 37: 224-236. PMID: 34664776
- The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex mediating autophagosome-lysosome fusion. | Jian, F., et al. 2024. Cell Res. 34: 151-168. PMID: 38182888
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAP47 Antibody (D-11) | sc-514428 | 200 µg/ml | $316.00 | |||
SNAP47 Antibody (D-11): m-IgG Fc BP-HRP Bundle | sc-527900 | 200 µg Ab; 10 µg BP | $354.00 | |||
SNAP47 Antibody (D-11): m-IgG1 BP-HRP Bundle | sc-533273 | 200 µg Ab; 20 µg BP | $354.00 | |||
SNAP47 Antibody (D-11) AC | sc-514428 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SNAP47 Antibody (D-11) HRP | sc-514428 HRP | 200 µg/ml | $316.00 | |||
SNAP47 Antibody (D-11) FITC | sc-514428 FITC | 200 µg/ml | $330.00 | |||
SNAP47 Antibody (D-11) PE | sc-514428 PE | 200 µg/ml | $343.00 | |||
SNAP47 Antibody (D-11) Alexa Fluor® 488 | sc-514428 AF488 | 200 µg/ml | $357.00 | |||
SNAP47 Antibody (D-11) Alexa Fluor® 546 | sc-514428 AF546 | 200 µg/ml | $357.00 | |||
SNAP47 Antibody (D-11) Alexa Fluor® 594 | sc-514428 AF594 | 200 µg/ml | $357.00 | |||
SNAP47 Antibody (D-11) Alexa Fluor® 647 | sc-514428 AF647 | 200 µg/ml | $357.00 | |||
SNAP47 Antibody (D-11) Alexa Fluor® 680 | sc-514428 AF680 | 200 µg/ml | $357.00 | |||
SNAP47 Antibody (D-11) Alexa Fluor® 790 | sc-514428 AF790 | 200 µg/ml | $357.00 |