SMIT Antibody (3A6): sc-293330

SMIT Antibody (3A6) is a mouse monoclonal IgG1 kappa light chain antibody that detects SMIT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-SMIT antibody (3A6) is available as the non-conjugated form. SMIT protein, encoded by the SLC5A3 gene located on chromosome 21q22.12, plays a crucial role in cellular regulation by facilitating myo-inositol uptake, a molecule essential for various cellular processes including membrane structure, signal transduction, and osmoregulation. SMIT protein helps maintain intracellular concentrations of myo-inositol, which is vital for cellular homeostasis, especially under hypertonic stress conditions where activity increases. SMIT protein is expressed in several human tissues, including brain, kidney, and placenta, with notable abundance in fetal brain and spinal cord, while expression diminishes in adult brain except in choroid plexus. SMIT protein localizes to basolateral membranes of thick ascending limb of Henle and inner medullary collecting duct, highlighting significance in renal function. Impaired SMIT function has been linked to diabetes and Down syndrome pathogenesis, making SMIT protein a critical target for research and therapeutic interventions.

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SMIT Antibody (3A6) References:

1. The human osmoregulatory Na+/myo-inositol cotransporter gene (SLC5A3): molecular cloning and localization to chromosome 21.  |  Berry, GT., et al. 1995. Genomics. 25: 507-13. PMID: 7789985
2. Localization and regulation of renal Na+/myo-inositol cotransporter in diabetic rats.  |  Wiese, TJ., et al. 1996. Kidney Int. 50: 1202-11. PMID: 8887279
3. Developmental regulation of Na+ / myo-inositol cotransporter gene expression.  |  Guo, W., et al. 1997. Brain Res Mol Brain Res. 51: 91-6. PMID: 9427510
4. The structural organization of the human Na+/myo-inositol cotransporter (SLC5A3) gene and characterization of the promoter.  |  Mallee, JJ., et al. 1997. Genomics. 46: 459-65. PMID: 9441750
5. Human Na(+)-myo-inositol cotransporter gene: alternate splicing generates diverse transcripts.  |  Porcellati, F., et al. 1998. Am J Physiol. 274: C1215-25. PMID: 9612208
6. Expression of the Na+/myo-inositol cotransporter in the juxtaglomerular region.  |  Yamauchi, A., et al. 1998. Kidney Int Suppl. 67: S183-5. PMID: 9736284

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SMIT Antibody (3A6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SMIT Antibody (3A6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SMIT Antibody (3A6) Specifications

Alternate Product Name: SMIT

Product Clone Name: 3A6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 533-641

Epitope Target: SMIT

Epitope Species: human

Product Reactivity/Detection: SMIT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SMIT Antibody (3A6): N/A