Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SMC6 Double Nickase Plasmid (h): sc-402805-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SMC6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SMC6 Double Nickase Plasmid (h) and SMC6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SMC6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SMC6 Antibody (A-3): sc-365742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMC6 Double Nickase Plasmid (h) | sc-402805-NIC | 20 µg | $410.00 | |||
SMC6 Double Nickase Plasmid (h2) | sc-402805-NIC-2 | 20 µg | $410.00 |
SMC6 encodes a core structural maintenance of chromosomes protein that partners with SMC5 to form the SMC5/6 complex, a key regulator of genome stability. This complex supports DNA replication and repair by coordinating homologous recombination, stabilizing stalled replication forks, and promoting resolution of DNA intermediates arising from replication stress. SMC6 also contributes to chromosome segregation and telomere maintenance, linking its activity to cell-cycle progression and preservation of chromosomal integrity. Disruption or dysregulation of SMC5/6 pathway components is associated with heightened genomic instability, a feature relevant to studies of DNA damage response defects and chromosome maintenance disorders.
SMC6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SMC6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SMC6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SMC6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SMC6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.