Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SMARCD2 Double Nickase Plasmid (h): sc-403091-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SMARCD2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SMARCD2 Double Nickase Plasmid (h) and SMARCD2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SMARCD2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SMARCD2 Antibody (F-34): sc-101162
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMARCD2 Double Nickase Plasmid (h) | sc-403091-NIC | 20 µg | $410.00 |
SMARCD2 (BAF60B) encodes a non-catalytic subunit of the SWI/SNF (BAF) ATP-dependent chromatin remodeling complex that modulates nucleosome positioning to regulate transcription, lineage specification, and chromatin accessibility. Through interactions with transcription factors and core BAF components, SMARCD2 contributes to programs governing hematopoietic differentiation, cell-cycle control, and developmental gene regulation. Dysregulation of SWI/SNF subunits, including SMARCD2, is linked to altered epigenetic states and transcriptional wiring observed in multiple cancers and other disorders of chromatin regulation. SMARCD2 therefore serves as a useful node for investigating chromatin remodeling mechanisms and context-dependent transcriptional networks in human cells.
SMARCD2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SMARCD2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SMARCD2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SMARCD2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SMARCD2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.