Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SMARCA4/Brg1 Double Nickase Plasmid (m): sc-423026-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SMARCA4/Brg1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SMARCA4/Brg1 Double Nickase Plasmid (m) and SMARCA4/Brg1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Smarca4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SMARCA4/Brg1 Antibody (G-7): sc-17796
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMARCA4/Brg1 Double Nickase Plasmid (m) | sc-423026-NIC | 20 µg | $410.00 | |||
SMARCA4/Brg1 Double Nickase Plasmid (m2) | sc-423026-NIC-2 | 20 µg | $410.00 |
Mouse Smarca4 encodes SMARCA4/Brg1, the ATPase catalytic subunit of SWI/SNF (BAF) chromatin remodeling complexes that reposition nucleosomes to regulate promoter and enhancer accessibility. Through ATP-dependent chromatin remodeling, SMARCA4 integrates with transcriptional programs controlling cell cycle progression, lineage specification, DNA replication, and DNA damage responses. SMARCA4/Brg1 function intersects with pathways governed by Polycomb repression, p53 signaling, and developmental transcription factors, shaping epigenetic states across tissues. Dysregulation of SWI/SNF components, including SMARCA4, is implicated in oncogenic transformation and developmental phenotypes, making Smarca4 a central target for mechanistic studies of chromatin-based gene regulation.
SMARCA4/Brg1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Smarca4 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Smarca4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Smarca4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Smarca4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.