SMARCA4/Brg1 Double Nickase Plasmid (h): sc-400168-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SMARCA4/Brg1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SMARCA4/Brg1 Double Nickase Plasmid (h) and SMARCA4/Brg1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SMARCA4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SMARCA4/Brg1 Antibody (G-7): sc-17796
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SMARCA4/Brg1 Double Nickase Plasmid (h) | sc-400168-NIC | 20 µg | $410.00 | |||
SMARCA4/Brg1 Double Nickase Plasmid (h2) | sc-400168-NIC-2 | 20 µg | $410.00 |
SMARCA4 (Brg1) encodes the ATPase catalytic core of the SWI/SNF (BAF) chromatin remodeling complex, which repositions nucleosomes to regulate transcriptional programs controlling lineage specification, cell-cycle progression, DNA damage responses, and differentiation. Through ATP-dependent chromatin remodeling, SMARCA4 influences enhancer accessibility and cooperates with sequence-specific transcription factors to modulate gene expression across developmental and stress-response pathways. Disruption of SMARCA4 perturbs chromatin state and genome regulation, and recurrent alterations are linked to aberrant epigenetic control and oncogenic transcriptional wiring in multiple tumor contexts. As a central chromatin regulator, SMARCA4 is widely studied in chromatin accessibility, transcriptional dependency mapping, and synthetic-lethality networks involving SWI/SNF subunits and Polycomb repression.
SMARCA4/Brg1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SMARCA4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SMARCA4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SMARCA4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SMARCA4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.