Sm D3 CRISPR/Cas9 KO Plasmid (h): sc-405302
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sm D3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sm D3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sm D3 CRISPR/Cas9 KO Plasmid (h) | sc-405302 | 20 µg | $397.00 |
Overview
SNRPD3 encodes the Sm D3 core protein, a conserved component of the Sm ring that assembles onto snRNAs to form spliceosomal small nuclear ribonucleoproteins (snRNPs). Sm D3 supports snRNP biogenesis and pre-mRNA splicing fidelity, linking it to spliceosome assembly dynamics and RNA processing quality control. Perturbation of Sm core proteins can shift transcript isoform usage and affect gene expression programs governing cell cycle progression, stress responses, and differentiation. Because aberrant splicing is a common feature of many disease states, SNRPD3 is frequently studied as a node connecting spliceosomal integrity to genome-wide RNA maturation phenotypes in human cells.
Sm D3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SNRPD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SNRPD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SNRPD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sm D3 protein expression.
This CRISPR knockout system enables efficient generation of SNRPD3-deficient cell models for investigation of Sm D3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SNRPD3 exon(s) critical for Sm D3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SNRPD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sm D3 CRISPR/Cas9 KO Plasmid (h) and Sm D3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SNRPD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sm D3 HDR Plasmid (h) and Sm D3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SNRPD3 homology arms to support homology-directed repair at defined SNRPD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.