Date published: 2026-1-9

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Sm B/B'/N Antibody (H-9): sc-374078

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Datasheets
  • Sm B/B'/N Antibody (H-9) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 1-240 representing full length SmB of human origin
  • recommended for detection of Sm B, Sm B′ and Sm N of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
  • m-IgGκ BP-HRP is the preferred secondary detection reagent for Sm B/B'/N Antibody (H-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with Sm B/B'/N Antibody (H-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

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Sm B/B′/N Antibody (H-9) is a mouse monoclonal IgG2b antibody that detects Sm B/B′/N in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Sm B/B′/N is a crucial component of the spliceosome, a complex responsible for processing mRNA precursors by excising introns to create continuous coding sequences. Sm B/B′/N plays a key role in spliceosome assembly and stability, ensuring proper mRNA splicing and gene expression regulation. Dysregulation of this process can lead to various diseases, including autoimmune disorders such as systemic lupus erythematosus, where anti-Sm B/B′/N antibodies are commonly produced. Anti-Sm B/B′/N antibody (H-9) provides a valuable tool for researchers studying mRNA processing and spliceosomal dysfunction in disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Sm B/B'/N Antibody (H-9)

sc-374078
200 µg/ml
$322.00

Sm B/B'/N Antibody (H-9): m-IgGκ BP-HRP Bundle

sc-552022
200 µg Ab; 40 µg BP
$361.00

Can Sm B/B'/N (H-9): sc-374078 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Sm B/B'/N (H-9): sc-374078 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Robust bands in western blot analysis ofRobust bands in western blot analysis of Sm B/B'/N expression in K-562, HeLa and KNRK nuclear extracts. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Superb immunoperoxidase nuclear stainingSuperb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date published: 2015-01-22
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Sm B/B'/N Antibody (H-9) is rated 5.0 out of 5 by 2.
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